Figure 3 | Oncogene

Figure 3

From: The role of tumor necrosis factor α in the pathophysiology of human multiple myeloma: therapeutic applications

Figure 3

TNFα induces NF-κB and p44/42 MAPK activation in BMSCs. (a) BMSCs were cultured with TNFα (0.1–10 ng/ml), and proliferation assessed at 48 h using MTT assay. Values represent the mean (+s.d.) of triplicate cultures. (b) Electrophoretic mobility shift assay using nuclear extract from BMSCs was performed as described in Materials and methods. BMSCs cells were pre-treated with either DMSO control or PS-341 (5 μM for 1 h) prior to stimulation with TNFα (10 ng/ml) for indicated intervals. Nuclear extracts from BMSCs were immunoblotted with anti-p65 NF-κB Ab. (c) BMSCs were stimulated with TNFα (10 ng/ml). Whole lysates at different intervals were immunoblotted with anti-phospho-MAPK or ERK1 Abs

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