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  • Oncogenomics
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Identification of AXUD1, a novel human gene induced by AXIN1 and its reduced expression in human carcinomas of the lung, liver, colon and kidney

Oncogene volume 20, pages 5062–5066 (2001)Cite this article

Abstract

Axin, an important regulator of β-catenin, is frequently mutated in human hepatocellular carcinomas (HCCs), and transduction of the wild-type Axin gene (AXIN1) induces apoptosis in HCC cells as well as in colon cancer cells. To investigate the detailed biological function of Axin, we searched on a cDNA microarray for genes whose expression was altered by transfer of wild-type AXIN1 into colon-cancer cell line LoVo. Among the genes showing altered expression, we focused on one, termed AXUD1 (AXIN1 up-regulated1), that revealed enhanced expression in response to exogeneously expressed AXIN1 but not to LacZ, a control gene. The AXUD1 gene consists of five exons and encodes a transcript with an open reading frame of 1767 bp. A 3.2-kb transcript of AXUD1 was expressed in all human tissues examined, most abundantly in lung, placenta, skeletal muscle, pancreas and leukocyte. By radiation-hybrid mapping we assigned its chromosomal location at 3p22, a region where frequent loss of heterozygosity has been reported in lung, renal, prostate, breast and cervical cancers. AXUD1 was frequently down-regulated in lung, kidney, liver and colon cancers compared with their corresponding normal tissues, suggesting that AXUD1 may have a tumor-suppressor function in those organs.

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Acknowledgements

We are grateful to Tae Makino for her technical assistance. This work was supported by a ‘Research for the Future’ Program Grant (96L00102) from the Japan Society for the Promotion of Science.

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Authors and Affiliations

  1. Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan

    Hideyuki Ishiguro, Yusuke Nakamura & Yoichi Furukawa

  2. Second Department of Surgery, Nagoya City University School of Medicine, 1 Kawasumi Mizuho-ku, Nagoya, 467-8601, Japan

    Hideyuki Ishiguro & Yoshitaka Fujii

  3. SNP Research Center, Riken (Institute of Physical and Chemical Research), 4-6-1, Shirokanedai, Minato-ku, 108-8639, Tokyo, Japan

    Tatsuhiko Tsunoda & Toshihiro Tanaka

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  1. Hideyuki Ishiguro
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  2. Tatsuhiko Tsunoda
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  3. Toshihiro Tanaka
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  4. Yoshitaka Fujii
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  6. Yoichi Furukawa
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Correspondence to Yoichi Furukawa.

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Ishiguro, H., Tsunoda, T., Tanaka, T. et al. Identification of AXUD1, a novel human gene induced by AXIN1 and its reduced expression in human carcinomas of the lung, liver, colon and kidney. Oncogene 20, 5062–5066 (2001). https://doi.org/10.1038/sj.onc.1204603

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