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Absence of PPP2R1A mutations in Wilms tumor

Oncogene volume 20, pages 2050–2054 (2001)Cite this article

Abstract

Evidence from genetic linkage analysis indicates that a gene located at 19q13.4, FWT2, is responsible for predisposition to Wilms tumor in many Wilms tumor families. This region has also been implicated in the etiology of sporadic Wilms tumor through loss of heterozygosity analyses. The PPP2R1A gene, encoding the α isoform of the heterotrimeric serine/threonine protein phosphatase 2A (PP2A), is located within the FWT2 candidate region and is altered in breast and lung carcinomas. PPP2R1B, encoding the β isoform, is mutated in lung, colon, and breast cancers. These findings suggested that both PPP2R1A and PPP2R1B may be tumor suppressor genes. Additionally, PP2A is important in fetal kidney growth and differentiation and has an expression pattern similar to that of the Wilms tumor suppressor gene WT1. Since PPP2R1A was therefore a compelling candidate for the FWT2 gene, we analysed the coding region of PPP2R1A in DNA and RNA samples from affected members of four Wilms tumor families and 30 sporadic tumors and identified no mutations in PPP2R1A in any of these 34 samples. We conclude that PPP2R1A is not the 19q familial Wilms tumor gene and that mutation of PPP2R1A is not a common event in the etiology of sporadic Wilms tumor.

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References

  • Afshari CA, Kodama S, Bivins HM, Willard TB, Fujiki H, Barrett JC . 1993 Cancer Res. 53: 1777–1782

  • Ashworth LK, Batzer MA, Brandriff B, Branscomb E, de Jong P, Garcia E, Garnes JA, Gordon LA, Lamerdin JE, Lennon G, Mohrenweiser H, Olsen AS, Slezak T, Carrano AV . 1995 Nature Genet. 11: 422–427

  • Bialojan C, Takai A . 1988 Biochem. J. 256: 283–290

  • Breslow N, Olshan A, Beckwith JB, Green DM . 1993 Med. Pediatr. Oncol. 21: 172–181

  • Calin GA, Di Iasio MG, Caprini E, Vorechovsky I, Natalia PG, Sozzi G, Croce CM, Barbanti-Brodano G, Russo G, Negrini M . 2000 Oncogene 19: 1191–1195

  • Call KM, Glaser T, Ito CY, Buckler AJ, Pelletier J, Haber DA, Rose EA, Kral A, Yeger H, Lewis WH, Jones C, Housman DE . 1990 Cell 60: 509–520

  • Depaoli-Roach AA, Park I-K, Cerovsky V, Csortos C, Durbin SD, Kuntz MJ, Sitikov A, Tang PM, Verin A, Zolnierowicz S . 1994 Advan. Enzyme Regul. 34: 199–224

  • Everett AD, Xue C, Stoops T . 1999 J. Am. Soc. Nephrol. 10: 1737–1745

  • Fagotto F, Jho Eh, Zeng L, Kurth T, Joos T, Kaufmann C, Constantini F . 1999 J. Cell. Biol. 145: 741–756

  • Gessler M, Poustka A, Cavenee W, Neve RL, Orkin SH, Bruns GA . 1990 Nature 343: 774–778

  • Grubb GR, Yun K, Williams BR, Eccles MR, Reeve AE . 1994 Lab. Invest. 71: 472–479

  • Hemmings BA, Adams-Pearson C, Maurer F, Muller P, Goris J, Merlevede W, Hofsteenge J, Stone SR . 1990 Biochemistry 29: 3166–3173

  • Hendrix P, Turowski P, Mayer-Jaeckel RE, Goris J, Hofsteenge J, Merlevede W, Hemmings BA . 1993 J. Biol. Chem. 268: 7330–7337

  • Hsu W, Zeng L, Costantini F . 1999 J. Biol. Chem. 274: 3439–3445

  • Huff V, Compton DA, Strong LC, Saunders GF . 1990 Hum. Genet. 84: 253–257

  • Huff V, Amos CI, Douglass ED, Fisher R, Geiser CF, Krill CE, Li FP, Strong LC, McDonald JM . 1997 Cancer Res. 57: 1859–1862

  • Ikeda S, Kishida M, Matsuura Y, Usui H, Kikuchi A . 2000 Oncogene 19: 537–545

  • Koesters R, Ridder R, Kopp-Schneider A, Betts D, Adams V, Niggli F, Briner J, von Knebel Doeberitz M . 1999 Cancer Res. 59: 3880–3882

  • Kohno T, Takahura S, Yamada T, Okamoto A, Tanaka T, Yokota J . 1999 Cancer Res. 59: 4170–4174

  • Li DM, Sun H . 1997 Cancer Res. 57: 2124–2129

  • Li J, Yen C, Liaw D, Podsypanina K, Bose S, Wang SI, Puc J, Miliaresis C, Rodgers L, McCombie R, Bigner SH, Giovanella BC, Ittmann M, Tycko B, Hibshoosh H, Wigler MH, Parsons R . 1997 Science 275: 1943–1947

  • Lin XH, Walter J, Scheidtmann K, Ohst K, Newport J, Walter G . 1998 Proc. Natl. Acad. Sci. USA 95: 14693–14698

  • Maiti S, Alam R, Amos CI, Huff V . 2000 Cancer Res. 60: 6288–6292

  • Mannens M, Devilee P, Bliek J, Mandjes I, de Kraker J, Heyting C, Slater RM, Westerveld A . 1990 Cancer Res. 50: 3279–3283

  • Maw MA, Grundy PE, Millow LJ, Eccles MR, Dunn RS, Smith PJ, Feinberg AP, Law DJ, Paterson MC, Telzerow PE, Callen DF, Thompson AD, Richards RI, Reeve AE . 1992 Cancer Res. 52: 3094–3098

  • Mayer-Jaeckel RE, Hemmings BA . 1994 Trends Cell Biol. 4: 287–291

  • McDonald JM, Douglass EC, Fisher R, Geiser CF, Krill CE, Strong LC, Virshup D, Huff V . 1998 Cancer Res. 58: 1387–1390

  • Miozzo M, Perotti D, Minoletti F, Mondini P, Pilotti S, Luksch R, Fossati-Bellani F, Pierotti MA, Sozzi G, Radice P . 1996 Genomics 37: 310–315

  • Miwa H, Tomlinson GE, Timmons CF, Huff V, Cohn SL, Strong LC, Saunders GF . 1992 J. Natl. Cancer Inst. 84: 181–187

  • Mumby MC, Walter G . 1993 Physiol. Rev. 73: 673–699

  • Pallas DC, Shahrik LK, Martin BL, Jaspers S, Miller TB, Brautigan DL, Roberts TM . 1990 Cell 60: 167–176

  • Pritchard-Jones K, Fleming S, Davidson D, Bickmore W, Porteous D, Gosden C, Bard J, Buckler A, Pelletier J, Housman D, van Heyningen V, Hastie N . 1990 Nature 346: 194–197

  • Rahman N, Arbour L, Tonin P, Renshaw J, Pelletier J, Baruchel S, Pritchard-Jones K, Stratton MR, Narod SA . 1996 Nat. Genet. 13: 461–463

  • Seeling JM, Miller JR, Gil R, Moon RT, White R, Virshup DM . 1999 Science 283: 2089–2091

  • Steck PA, Pershouse MA, Jasser SA, Yung WK, Lin H, Ligon AH, Langford LA, Baumgard ML, Hattier T, Davis T, Frye C, Hu R, Swedlund B, Teng DH, Tavtigian SV . 1997 Nat. Genet. 15: 356–362

  • Suganuma M, Fujiki H, Suguri H, Yoshizawa S, Hirota M, Nakayasu M, Ojika M, Wakamatsu K, Yamada K, Sugimura T . 1988 Proc. Natl. Acad. Sci. USA 85: 1768–1771

  • Svennilson J, Durbeej M, Celsi G, Laestadius A, da Cruz e Silva EF, Ekblom P, Aperia A . 1995 Kidney Int. 48: 103–110

  • Takakura S, Kohno T, Shimizu K, Ohwada S, Okamoto A, Yokota J . 2000 Oncogene 19: 836–840

  • Virshup D . 2000 Curr. Opin. Cell Biol. 12: 180–185

  • Walter G, Ferre F, Espiritu O, Carbone-Wiley A . 1989 Proc. Natl. Acad. Sci. USA 86: 8669–8672

  • Walter G, Ruediger R, Slaughter C, Mumby M . 1990 Proc. Natl. Acad. Sci. USA 87: 2521–2525

  • Wang SS, Esplin ED, Li JL, Huang L, Gazdar A, Minna J, Evans GA . 1998 Science 282: 284–287

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Acknowledgements

The cooperation and help of patients and their physicians is greatly appreciated. We thank R Alam for excellent technical assistance and P Begin and D Sembera for sample collection. This work was supported in part by NCI grants CA78257, CA34936, CA16672, and a grant from the Kadoorie Foundation. A portion of this work was performed under the auspices of the US Department of Energy at Lawrence Livermore National Laboratory under Contract No. W-7405-ENG-48.

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  1. Department of Experimental Pediatrics, The University of Texas MD Anderson Cancer Center, 77030, Houston, Texas, TX, USA

    E Cristy Ruteshouser & Vicki Huff

  2. Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, 94551, California, CA, USA

    Linda K Ashworth

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  2. Linda K Ashworth
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Ruteshouser, E., Ashworth, L. & Huff, V. Absence of PPP2R1A mutations in Wilms tumor. Oncogene 20, 2050–2054 (2001). https://doi.org/10.1038/sj.onc.1204301

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