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A monoclonal antibody against DNA binding helix of p53 protein

Oncogene volume 20, pages 1398–1401 (2001)Cite this article

Abstract

Three monoclonal antibodies (Mabs) were generated against p53 DNA-binding core domain. When tested by immunoprecipitation, Western blot and immunofluorescence techniques, Mab 9E4, as well as 7D3 and 6B10 reacted with both wild-type and various mutant p53 proteins. The epitopes recognized by Mabs 7D3, 9E4 and 6B10 were located respectively within the amino acid residues 211-220, 281-290 and 291-300 of human p53 protein. The epitope recognized by 9E4 Mab coincides with helix 2, also called p53 DNA binding helix, which allows the direct contact of the protein with its target DNA sequences. This antibody may be useful to study transcription-dependent and transcription-independent activities of wild-type and mutant p53 proteins.

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Acknowledgements

This work is supported by grants from ICGEB and TUBITAK.

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Authors and Affiliations

  1. Department of Molecular Biology and Genetics and BilGen Genetics and Biotechnology Center, Bilkent University, Ankara, 06533, Turkey

    Esma Yolcu, Berna S Sayan, Rengul Cetin-Atalay & Mehmet Ozturk

  2. Department of Molecular Oncology, TUBITAK-MRC, Research Institute for Genetic Engineering and Biotechnology, Gebze, Kocaeli, Turkey

    Esma Yolcu, Tamer Yağci, Nevzat Yurdusev & Mehmet Ozturk

  3. Institut Curie, Paris, France

    Thierry Soussi

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  1. Esma Yolcu
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  2. Berna S Sayan
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Yolcu, E., Sayan, B., Yağci, T. et al. A monoclonal antibody against DNA binding helix of p53 protein. Oncogene 20, 1398–1401 (2001). https://doi.org/10.1038/sj.onc.1204240

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