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A nude mice model of human rhabdomyosarcoma lung metastases for evaluating the role of polysialic acids in the metastatic process

Oncogene volume 20, pages 997–1004 (2001)Cite this article

Abstract

PSA is an oncodevelopmental antigen usually expressed in human tumors with high metastatic potential. Here we set up a metastatic model in nude mice by using TE671 cells, which strongly express PSA-NCAM. We observed the formation of lung metastases when TE671 cells were injected intravenously, intramuscularly, and intraperitoneously, but not subcutaneously. Intraperitoneous injections also induced peritoneal carcinosis, ascites, and liver metastases. To evaluate the putative role of PSA in the metastatic process we used a specific cleavage of PSA on NCAM by endoneuraminidase-N on intraperitoneous primary tumors. Mice with primary intramuscular tumors were taken as control. Repeated injections of endoneuraminidase-N led to a decrease in PSA expression in primary intraperitoneous nodules and ascites but not in intramuscular primary tumors. Endoneuraminidase-N also increased the delay in ascitic formation and decreased the number of lung or liver metastases in the case of intraperitoneous tumors but not in the case of intramuscular tumors. When metastases occurred in endoneuraminidase-N injected animals, they strongly expressed PSA-NCAM. Therefore, we established a relationship between PSA expression on the surface of primary tumor cells and the metastatic process.

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Acknowledgements

We thank J Barbet and JF Pellissier for comments, J Gouvernet for statistical analyses, and G Burkhart for technical assistance. The karyotypic analysis of TE 671 cell line was performed by H Zattara-Cannoni (Service de Cytogénétique, Timone Hospital, Marseille, France). This work was supported by grants from the ‘Association pour la Recherche contre le Cancer’ (to D Figarella-Branger and to G Rougon), the GEFLUC (Groupement des Entreprises Françaises dans la Lutte contre le Cancer) (to D Figarella-Branger), PHRC (to D Figarella-Branger) and institutional grants (to JE 2053).

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Authors and Affiliations

  1. Laboratoire de Biopathologie Nerveuse et Musculaire (JE2053). Institut de Biologie du Développement de Marseille, Faculté de Médecine, 27 Bd Jean Moulin 13385, Marseille, 05, Cedex, France

    Laurent Daniel & Dominique Figarella-Branger

  2. Laboratoire de Génétique et de Physiologie du Développement, CNRS, campus de Luminy case 907, 13288, Marseille, 09, Cedex, France

    Pascale Durbec & Geneviève Rougon

  3. Département Imagerie et Thérapeutique, Immunotech S.A. 130, av De Lattre de Tassigny, Marseille, 13009, France

    Emmanuel Gautherot & Eric Rouvier

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  1. Laurent Daniel
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Daniel, L., Durbec, P., Gautherot, E. et al. A nude mice model of human rhabdomyosarcoma lung metastases for evaluating the role of polysialic acids in the metastatic process. Oncogene 20, 997–1004 (2001). https://doi.org/10.1038/sj.onc.1204176

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