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Glial tumor cell adhesion is mediated by binding of the FNIII domain of receptor protein tyrosine phosphatase β (RPTPβ) to tenascin C

Oncogene volume 20, pages 609–618 (2001)Cite this article

Abstract

The extracellular domain of receptor protein tyrosine phosphatase β (RPTPβ) is composed of several domains which mediate its interactions with distinct ligands present on the surface of either neurons or glial cells. Here, we demonstrate that the fibronectin type III domain (FNIII) of RPTPβ binds to glial tumor-derived cell lines and primary astrocytes. We used affinity purification to isolate several proteins that specifically bind to the FNIII domain of RPTPβ. One of these, a 240 kDa protein that was purified from U118MG glioblastoma cell, was identified as tenascin C based on the amino acid sequence of several tryptic peptides. The interaction of RPTPβ with tenascin C was found to mediate cell adhesion. Adhesion and spreading of SF763T astrocytoma cells expressing RPTPβ on tenascin C was specifically abolished by the addition of a soluble fragment containing the FNIII domain of the receptor. RPTPβ-dependent cell adhesion was mediated by binding to the alternatively spliced FNIII repeats A1,2,4 (TnfnA1,2,4) of tenascin C. Furthermore, COS cells expressing RPTPβ adhere to TnfnA1,2,4, while the parental cells did not. These results demonstrate that the FNIII domain of RPTPβ binds to tenascin C and suggest that RPTPβ present on glial tumor cells is a primary adhesion receptor system to the extracellular matrix.

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Acknowledgements

This work was supported by the Dorot foundation from the Israel Academy of Sciences and Humanities, the Yad Abraham Research Center for Cancer Diagnostics and Therapy, and a Research Career Development Award from the Israel Cancer Research Fund. E Peles is an Incumbent of the Madeleine Haas Russell Career Development Chair.

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Authors and Affiliations

  1. Department of Molecular Cell Biology, The Weizmann Institute of Science, Rehovot, 76100, Israel

    Konstantin Adamsky & Elior Peles

  2. Sugen Inc., South San Francisco, CA 94080, California, USA

    James Schilling

  3. Unité CNRS UPR 1352, Centre de Neurochimie, Strasbourg, 67084, France

    Jeremy Garwood & Andreas Faissner

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  1. Konstantin Adamsky
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Adamsky, K., Schilling, J., Garwood, J. et al. Glial tumor cell adhesion is mediated by binding of the FNIII domain of receptor protein tyrosine phosphatase β (RPTPβ) to tenascin C. Oncogene 20, 609–618 (2001). https://doi.org/10.1038/sj.onc.1204119

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