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Functional evidence for an ovarian cancer tumor suppressor gene on chromosome 22 by microcell-mediated chromosome transfer

Oncogene volume 19, pages 6277–6285 (2000)Cite this article

Abstract

The identity of many tumor suppressor genes important in epithelial ovarian cancer tumorigenesis remains unknown. In an effort to localize a novel tumor suppressor on chromosome 22, a psv2neo tagged human chromosome 22 was transferred into the malignant epithelial ovarian cancer cell line, SKOv-3, by microcell-mediated chromosome transfer. Complete suppression of the transformed phenotype was observed in 16 of 18 individual microcell hybrid clones as evidenced by the complete abrogation of cell growth under anchorage-independent conditions. In vitro doubling times were also dramatically reduced, as was the ability to form subcutaneous tumors in CD1 nu/nu mice. Only one polymorphic marker, D22S429, segregated with decreased transformation and tumorigenic potential, suggesting that an unrecognized tumor suppressor may localize to chromosome 22q11-q12. These data provide functional support for the presence of a novel tumor suppressor locus (or loci) on chromosome 22 that is important in ovarian cancer tumorigenesis.

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Acknowledgements

We would like to express our thanks to the laboratory of M Siciliano for their technical assistance during cytogenetic analyses. This study was supported by the Ovarian Cancer Research Fund.

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Authors and Affiliations

  1. Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX 77030, Texas, USA

    Russell P Kruzelock

  2. Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, Texas, USA

    Russell P Kruzelock, Yofre Cabeza-Arvelaiz & Mark Pershouse

  3. Department of Molecular Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, Texas, USA

    Bruce D Cuevas & Gordon B Mills

  4. Department of Clinical Investigation, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, Texas, USA

    Feng-Ji Xu, Yinhua Yu & Robert C Bast Jr

  5. Division of Medicine, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, Texas, USA

    Bruce D Cuevas, Feng-Ji Xu, Yinhua Yu, Gordon B Mills & Robert C Bast Jr

  6. Division of Laboratory Medicine, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, Texas, USA

    M Mercedes Lovell & Ann M Killary

  7. Graduate School of Biomedical Sciences, Houston, TX 77225, Texas, USA

    Jon R Wiener

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Kruzelock, R., Cuevas, B., Wiener, J. et al. Functional evidence for an ovarian cancer tumor suppressor gene on chromosome 22 by microcell-mediated chromosome transfer. Oncogene 19, 6277–6285 (2000). https://doi.org/10.1038/sj.onc.1204013

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