Abstract
The small GTPase Rho and one of its targets, Rho-kinase (also termed ROK or ROCK), are implicated in various cellular functions including stress fiber formation, smooth muscle contraction, tumor cell invasion and cell motility. We have previously reported that Rho-kinase accumulates at the cleavage furrow during cytokinesis in several cultured cells. Here, using Rho-kinase inhibitors, Y-27632 and HA1077, we found that Rho-kinase is responsible for the phosphorylation of myosin regulatory light chain at Ser19 in the cleavage furrow during cytokinesis. On the other hand, phosphorylation of ezrin/radixin/moesin (ERM) proteins at the cleavage furrow was enhanced by the addition of the above Rho-kinase inhibitors. Treatment with Y-27632 strongly enhanced the accumulation of Rho-kinase but not RhoA and citron kinase at the cleavage furrow. Furthermore, the furrow ingression in cytokinesis was significantly prolonged in the presence of Y-27632. These results suggest that Rho-kinase is involved in the progression of cytokinesis through the phosphorylation of several proteins including myosin light chain at the cleavage furrow.
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References
Amano M, Ito M, Kimura K, Fukata Y, Chihara K, Nakano T, Matsuura Y and Kaibuchi K. . 1996 J. Biol. Chem. 271: 20246–20249.
Fukata Y, Kimura K, Oshiro N, Saya H, Matsuura Y and Kaibuchi K. . 1998 J. Cell Biol. 141: 409–418.
Gerisch G and Weber I. . 2000 Curr. Opin. Cell Biol. 12: 126–132.
Hales KG, Bi E, Wu J-Q, Adam JC, Yu I-C and Pringle JR. . 1999 Curr. Opin. Cell Biol. 11: 717–725.
Hall A. . 1998 Science 279: 509–514.
Hayashi K, Yonemura S, Matsui T, Tsukita Sa and Tsukita Sh. . 1999 J. Cell Sci. 112: 1149–1158.
Huttenlocher A, Sandborg RR and Horwitz A. . 1995 Curr. Opin. Cell Biol. 7: 697–706.
Kaibuchi K, Kuroda S and Amano M. . 1999 Annu. Rev. Biochem. 68: 459–486.
Kimura K, Ito M, Amano M, Chihara K, Fukata Y, Nakafuku M, Yamamori B, Feng J, Nakano T, Okawa K, Iwamatsu A and Kaibuchi K. . 1996 Science 273: 245–248.
Kimura K, Fukata Y, Matsuoka Y, Bennett V, Matsuura Y, Okawa K, Iwamatsu A and Kaibuchi K. . 1998 J. Biol. Chem. 273: 5542–5548.
Kosako H, Goto H, Yanagida M, Matsuzawa K, Fujita M, Tomono Y, Okigaki T, Odai H, Kaibuchi K and Inagaki M. . 1999 Oncogene 18: 2783–2788.
Lim L, Manser E, Leung T and Hall C. . 1996 Eur. J. Biochem. 242: 171–185.
Madaule P, Eda M, Watanabe N, Fujisawa K, Matsuoka T, Bito H, Ishizaki T and Narumiya S. . 1998 Nature 394: 491–494.
Mangeat P, Roy C and Martin M. . 1999 Trends Cell Biol. 9: 187–192.
Matsui T, Maeda M, Doi Y, Yonemura S, Amano M, Kaibuchi K, Tsukita Sa and Tsukita Sh. . 1998 J. Cell Biol. 140: 647–657.
Matsui T, Yonemura S, Tsukita Sh and Tsukita Sa. . 1999 Curr. Biol. 9: 1259–1262.
Matsumura F, Ono S, Yamakita Y, Totsukawa G and Yamashiro S. . 1998 J. Cell Biol. 140: 119–129.
Narumiya S, Ishizaki T and Watanabe N. . 1997 FEBS Lett. 410: 68–72.
O'Connell CB, Wheatley SP, Ahmed S and Wang YL. . 1999 J. Cell Biol. 144: 305–313.
Oshiro N, Fukata Y and Kaibuchi K. . 1998 J. Biol. Chem. 273: 34663–34666.
Takai Y, Sasaki T, Tanaka K and Nakanishi H. . 1995 Trends Biochem. Sci. 20: 227–231.
Takaishi K, Sasaki T, Kameyama T, Tsukita Sa, Tsukita Sh and Takai Y. . 1995 Oncogene 11: 39–48.
Tsukita Sa and Yonemura S. . 1999 J. Biol. Chem. 274: 34507–34510.
Uehata M, Ishizaki T, Satoh H, Ono T, Kawahara T, Morishita T, Tamakawa H, Yamagami K, Inui J, Maekawa M and Narumiya S. . 1997 Nature 389: 990–994.
Van Aelst L and D'Souza-Schorey C. . 1997 Genes Dev. 11: 2295–2322.
Watanabe N, Madaule P, Reid T, Ishizaki T, Watanabe G, Kakizuka A, Saito Y, Nakao K, Jockusch BM and Narumiya S. . 1997 EMBO J. 16: 3044–3056.
Yasui Y, Amano M, Nagata K, Inagaki N, Nakamura H, Saya H, Kaibuchi K and Inagaki M. . 1998 J. Cell Biol. 143: 1249–1258.
Acknowledgements
We are grateful to Drs S Yonemura, Sa Tsukita and Sh Tsukita for providing us with anti-CP-ERMs mAb (297S). H Kosako thanks Dr M Nakafuku for encouragement and support. This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan, Japan Society of the Promotion of Science Research for the Future and a grant from Bristol-Myers-Squibb.
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Kosako, H., Yoshida, T., Matsumura, F. et al. Rho-kinase/ROCK is involved in cytokinesis through the phosphorylation of myosin light chain and not ezrin/radixin/moesin proteins at the cleavage furrow. Oncogene 19, 6059–6064 (2000). https://doi.org/10.1038/sj.onc.1203987
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DOI: https://doi.org/10.1038/sj.onc.1203987
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