Figure 4 | Oncogene

Figure 4

From: Up-regulation of hypoxia-inducible factors HIF-1α and HIF-2α under normoxic conditions in renal carcinoma cells by von Hippel-Lindau tumor suppressor gene loss of function

Figure 4

Expression of HIF-1α protein in different VHL deficient and VHL wildtype expressing RCC cell lines. Cells were cultured in parallel in normoxia (N) or hypoxia (H) for 5 h. Immunoblots of RCC whole cell extracts (100 μg) with HIF-1α antibody (Novus). (a) Lanes 1, 2, 9, 10: VHL deficient cell lines RCC6 and RCC10, lanes 3–8: VHL wildtype cell lines RCC7, RCC8 and RCC9. (b) Lanes 1, 2: VHL minus cell line RCC10, lanes 3–8: stably transfected, VHL wildtype expressing cell clones RCC10wt69, RCC10wt90 and RCC10wt63

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