Figure 3 | Oncogene

Figure 3

From: Up-regulation of hypoxia-inducible factors HIF-1α and HIF-2α under normoxic conditions in renal carcinoma cells by von Hippel-Lindau tumor suppressor gene loss of function

Figure 3

Northern blot analysis of total RNA from seven different human renal clear cell carcinoma cell lines: VHL deficient cell lines (mut) RCC2, RCC6, RCC10 and RCC11; VHL wildtype cell lines (wt) RCC7, RCC8 and RCC9. Hypoxic stimulation of the cells was carried out for 5 h. Ten μg RNA was loaded per lane. The blot was hybridized with 32P-labeled human VHL, VEGF, HIF-1α, HIF-2α and Glut1 probes. Hybridization with β-actin served as loading control

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