Figure 2 | Oncogene

Figure 2

From: Up-regulation of hypoxia-inducible factors HIF-1α and HIF-2α under normoxic conditions in renal carcinoma cells by von Hippel-Lindau tumor suppressor gene loss of function

Figure 2

Northern blot analysis of total RNA from VHL deficient cell line RCC10 and 11 stably transfected, VHL wildtype expressing cell clones. Lane 1: RCC10 (VHL minus), lanes 2–12: VHL wildtype cell clones RCC10wt53, RCC10wt63, RCC10wt64, RCC10wt69, RCC10wt71, RCC10wt84, RCC10wt90, RCC10wt92, RCC10wt106, RCC10wt115 and RCC10wt119. Ten μg RNA were loaded per lane. The blot was hybridized with 32P-labeled human VHL, VEGF, HIF-1α, HIF-2α and Glut1 probes. Hybridization with β-actin served as loading control

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