Figure 2 | Oncogene

Figure 2

From: A comparative evaluation of β-catenin and plakoglobin signaling activity

Figure 2

Expression, stability, and transactivation potential of wild type and mutant β-catenin and plakoglobin. (a) Western blots to determine cytoplasmic levels of β-catenin and plakoglobin. Top panel measures β-catenin levels 48 h after transfection of 293T cells with indicated amounts (in μg) of wild-type (WT) or mutant β-catenin DNA (S37A). The bottom panel shows cytoplasmic plakoglobin in 293T cells transiently transfected with the indicated amount (in μg) of wild type and mutant plakoglobin expression constructs (see Materials and methods). (b) Pulse/chase analysis and plakoglobin proteins. Shown are the per cent of protein at each time point relative to the start of the chase period. (c) Relative fold activation of the TO reporter induced by increasing amounts of the indicated plasmids after transient transfection of 293T cells

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