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Bcl-2 accelerates multistep prostate carcinogenesis in vivo

Oncogene volume 19, pages 5251–5258 (2000)Cite this article

Abstract

The impact of bcl-2 proto-oncogene expression on the pathogenesis and progression of prostate cancer was examined in a transgenic mouse model. Probasin-bcl-2 transgenic mice were crossed with TRAMP (TRansgenic Adenocarcinoma Mouse Prostate) mice that express the SV40 early genes (T/t antigens) under probasin control. Prostate size, cell proliferation, apoptosis, and the incidence and latency of tumor formation were evaluated. The double transgenic, probasin-bcl-2 X TRAMP F1 (BxT) mice exhibited an increase in the wet weight of the prostate. This was associated with an increase in proliferation, attributable to T/t antigens, and a decrease in apoptosis attributable to bcl-2. The latency to tumor formation was also decreased in the BxT mice compared to the TRAMP mice. The incidence of metastases was identical in both the TRAMP and BxT mice. Lastly, the incidence of hormone-independent prostate cancer was reduced in the BxT mice compared to the TRAMP mice. Together, these results demonstrate that bcl-2 can facilitate multistep prostate carcinogenesis in vivo.

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Acknowledgements

This work was supported by a grant from the American Cancer Society (RPG-96-036-04-CDD), an award from CaP CURE (The Association for the Cure of Cancer of the Prostate), NIH CA58204 (to NM Greenberg), and NIH Training Grant T32 CA67759-01 (EM Bruckheimer).

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Authors and Affiliations

  1. Department of Molecular Pathology, The University of Texas M.D. Anderson Cancer Center, Houston, 77030, Texas, TX, USA

    Elizabeth M Bruckheimer, Shawn Brisbay & Timothy J McDonnell

  2. Department of Biomathematics, The University of Texas M.D. Anderson Cancer Center, Houston, 77030, Texas, TX, USA

    Dennis J Johnson

  3. Department of Cell Biology, Baylor College of Medicine, Houston, 77030, Texas, TX, USA

    Jeffrey R Gingrich & Norman Greenberg

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  1. Elizabeth M Bruckheimer
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Bruckheimer, E., Brisbay, S., Johnson, D. et al. Bcl-2 accelerates multistep prostate carcinogenesis in vivo. Oncogene 19, 5251–5258 (2000). https://doi.org/10.1038/sj.onc.1203881

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