Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Short Report
  • Published:

Identification of negative regulatory regions within the first exon and intron of the BCL6 gene

Oncogene volume 19, pages 4941–4945 (2000)Cite this article

Abstract

Chromosomal translocations involving BCL6 gene are frequent in human B-cell lymphomas. Chromosomal breaks preferentially occur within a 3-kb region containing the first exon and intron. Recent reports have revealed that internal deletions or point mutations also are common in this region, suggesting that structural alteration of this region may be a crucial event in the development of lymphomas. In this study, we identified two regions in the BCL6 gene that negatively regulate BCL6 expression. One region, ES, is located within the first exon between nucleotides +472 and +543, and a second region, IS, is located between +783 and +918 of the first intron. A consensus nucleotide sequence for the binding of the BCL6 protein itself was found within the ES region. An electrophoretic mobility shift assay and a co-transfection experiment using a BCL6 expression vector showed that transcription of the BCL6 gene was negatively regulated by the BCL6 gene product. The IS region which is included in the regions commonly deleted in B-cell lymphomas had a silencer activity. Structural alterations of these two regions may play roles in the deregulated expression of the BCL6 gene in B-cell lymphomas.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on SpringerLink
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2
Figure 3

Similar content being viewed by others

References

  • Albagli O, Dhordain P, Bernardin F, Quief S, Kerkaert JP and Leprince D. . 1996 Biochem. Biophys. Res. Commun. 220: 911–915.

  • Baron BW, Nucifora G, McCabe N, Espinosa RI, Le Beau MM and McKeithan TW. . 1993 Proc. Natl. Acad. Sci. USA 90: 5262–5266.

  • Bernardin F, Collyn dHM, Quief S, Bastard C, Leprince D and Kerckaert JP. . 1997 Oncogene 14: 849–855.

  • Capello D, Carbone A, Pastore C, Gloghini A, Saglio G and Gaidano G. . 1997 Br. J. Haematol. 99: 168–170.

  • Cattoretti G, Zhang J, Cleary AM, Lederman S, Gaidano G, Carbone A and Chaganti RSK. . 1997 Blood 90: 175a.

  • Chang CC, Ye BH, Chaganti RS and Dalla-Favera R. . 1996 Proc. Natl. Acad. Sci. USA 93: 6947–6952.

  • Chen WY, Butler M, Rao PH, Chaganti SR, Louie DC, Dalla-Favera R and Chaganti RSK. . 1998 Oncogene 17: 1717–1722.

  • Deweindt C, Albagli O, Bernardin F, Dhordain P, Quief S, Lantoine D, Kerckaert JP and Leprince D. . 1995 Cell Growth Differ. 6: 1495–1503.

  • Gaidano G, Carbone A, Pastore C, Capello D, Migliazza A, Gloghini A, Roncella S, Ferrarini M, Saglio G and Dalla-Favera R. . 1997 Blood 89: 3755–3762.

  • Kawamata N, Miki T, Ohashi K, Suzuki K, Fukuda T, Hirosawa S and Aoki N. . 1994 Biochem. Biophys. Res. Commun. 204: 366–374.

  • Kerckaert JP, Deweindt C, Tilly H, Quief S, Lecocq G and Bastard C. . 1993 Nature Genet. 5: 66–70.

  • Klaus GG, Choi MS, Lam EW, Johnson-Leger C and Cliff J. . 1997 Int. Rev. Immunol. 15: 5–31.

  • Liang R, Chan WP, Kwong YL, Chan AC, Xu WS and Srivastava G. . 1998 Cancer. Genet. Cytogenet. 102: 110–113.

  • Migliazza A, Martinotti S, Chen W, Fusco C, Ye BH, Knowles DM, Offit K, Chaganti RS and Dalla-Favera R. . 1995 Proc. Natl. Acad. Sci. USA 92: 12520–12524.

  • Miki T, Kawamata N, Hirosawa S and Aoki N. . 1994 Blood 83: 26–32.

  • Nakamura Y, Miki T, Miura I, Hashimoto K, Miura A, Akimoto K, Hirosawa S, Saito K, Enokihara H, Furusawa S and Shishido H. . 1996 Leukemia 10: 658–661.

  • Nakamura Y, Saito K and Furusawa S. . 1999 Br. J. Haematol. 105: 274–277.

  • Ohashi K, Miki T, Hirosawa S and Aoki N. . 1995 Biochem. Biophys. Res. Commun. 214: 461–467.

  • Pasqualucci L, Migliazza A, Fracchiolla N, William C, Neri A, Baldini L, Chaganti RSK, Klein U, Kuppers R, Rajewsky K and Dalla-Favera R. . 1998 Proc. Natl. Acad. Sci. USA 95: 11816–11821.

  • Seyfert VL, Allman D, He Y and Staudt LM. . 1996 Oncogene 12: 2331–2342.

  • Shen HM, Peters A, Baron B, Zhu XD and Storb U. . 1998 Science 280: 1750–1752.

  • Ye BH, Lista F, Lo CF, Knowles DM, Offit K, Chaganti RS and Dalla-Favera R. . 1993 Science 262: 747–750.

Download references

Acknowledgements

We thank Dr Patrick Matthius for providing us with pKappaLucE and pH2BLucE, and Dr Hiroshi Itoh for pEFLac. We also thank Miss Kaori Okada for technical support. This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan.

Author information

Authors and Affiliations

  1. The School of Allied Health Science, Faculty of Medicine, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8519, Japan

    Mika Kikuchi & Ryuichi Kamiyama

  2. The First Department of Internal Medicine, Faculty of Medicine, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8519, Japan

    Tohru Miki, Takashi Kumagai, Tetsuya Fukuda, Nobuyuki Miyasaka & Shinsaku Hirosawa

Authors
  1. Mika Kikuchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tohru Miki
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Takashi Kumagai
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Tetsuya Fukuda
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ryuichi Kamiyama
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Nobuyuki Miyasaka
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Shinsaku Hirosawa
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kikuchi, M., Miki, T., Kumagai, T. et al. Identification of negative regulatory regions within the first exon and intron of the BCL6 gene. Oncogene 19, 4941–4945 (2000). https://doi.org/10.1038/sj.onc.1203864

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.onc.1203864

Keywords

This article is cited by

Search

Advanced search

Quick links