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Cloning and expression of human B cell-specific transcription factor BACH2 mapped to chromosome 6q15

Oncogene volume 19, pages 3739–3749 (2000)Cite this article

Abstract

The transcription factor Bach2, a member of the BTB-basic region leucine zipper (bZip) factor family, binds to a 12-O-tetradecanoylphorbol-13-acetate (TPA)-responsive element and the related Maf-recognition element (MARE) by forming homodimers or heterodimers with Maf-related transcription factors. Bach2 regulates transcription by binding to these elements. To understand the function in hematopoiesis, we isolated a cDNA clone for human Bach2 (BACH2) encoding a protein of 841 amino acid residues with a deduced amino acid sequence having 89.5% identity to mouse homolog. Among human hematopoietic cell lines, BACH2 is expressed abundantly only in some B-lymphocytic cell lines. RT–PCR analysis of hematopoietic cells revealed that BACH2 mRNA is expressed in primary B-cells. Enforced expression of BACH2 in a human Burkitt cell line, RAJI that does not express endogenous BACH2, resulted in marked reduction of clonogenic activity, indicating that BACH2 possesses an inhibitory effect on cell proliferation. By fluorescent in situ hybridization, the BACH2 gene was localized to chromosome 6q15. Because deletion of the long arm of chromosome 6 (6q) is one of the commonest chromosomal alterations in human B-cell lymphoma, we examined for the loss of heterozygosity (LOH) of the BACH2 gene in human B-cell non-Hodgkin's lymphomas (NHL). Among 25 informative cases, five (20%) showed LOH. These results indicate that BACH2 plays important roles in regulation of B cell development.

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Acknowledgements

We would like to dedicate this paper to Professor M Yokoyama who passed away on 11 March 2000. We thank Dr K Ohtani, Fujisaki Cell Center of Hayashibara Biochemical Laboratories, Inc., Dr Y Hayashi for various cell lines and helpful discussions, and Dr T Ikenaka for retrovirus vector and cell lines. This work was supported by Grants-in-Aid for Scientific Research, Grants-in-Aid for Scientific Research on Priority Areas and a Grant-in-Aid for Encouragement of Young Scientists from the Ministry of Education, Science, Sports and Culture and grants from Uehara Memorial Foundation and Naito Foundation.

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Author notes

  1. Tatsutoshi Nakahata

    Present address: Department of Pediatrics, Kyoto University, Faculty of Medicine, Kyoto, 606-8501, Japan

  2. Kazuhiko Igarashi

    Present address: Department of Biochemistry, Hiroshima University School of Medicine, Hiroshima, 734-8551, Japan

Authors and Affiliations

  1. Department of Pediatrics, School of Medicine, Hirosaki University, Hirosaki, 036-8563, Japan

    Shinya Sasaki, Etsuro Ito, Tsutomu Toki, Rika Kanezaki, Takamichi Umenai & Masaru Yokoyama

  2. Department of Biology, Faculty of Sciences, Hirosaki University, Hirosaki, 036-8563, Japan

    Rika Kanezaki

  3. Department of Transfusion Medicine and Cell Therapy, The Institute of Medical Science, The University of Tokyo, Minato-ku, 113-0033, Tokyo, Japan

    Taira Maekawa & Tatsutoshi Nakahata

  4. Center for Tsukuba Advanced Research Alliance and Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, 305-8575, Japan

    Akihiko Muto & Masayuki Yamamoto

  5. Department of Biochemistry, Tohoku University School of Medicine, Sendai, 980-8575, Japan

    Akihiko Muto & Kazuhiko Igarashi

  6. Department of Clinical Oncology, The Institute of Medical Science, The University of Tokyo, Minato-ku, 113-0033, Tokyo, Japan

    Tatsutoshi Nakahata

  7. First Department of Internal Medicine, Nagoya University School of Medicine, Nagoya, 466-8560, Japan

    Hirokazu Nagai & Tomohiro Kinoshita

  8. Department of Molecular Genetics, Division of Genetics, Medical Research Institute, Tokyo Medical and Dental University, Bunkyo-ku, 113-8519, Tokyo, Japan

    Johji Inazawa

  9. Graduate School of Pharmaceutical Sciences, The University of Tokyo, Bunkyo-ku, 113-0033, Tokyo, Japan

    Makoto M Taketo

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  1. Shinya Sasaki
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Sasaki, S., Ito, E., Toki, T. et al. Cloning and expression of human B cell-specific transcription factor BACH2 mapped to chromosome 6q15. Oncogene 19, 3739–3749 (2000). https://doi.org/10.1038/sj.onc.1203716

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  • DOI: https://doi.org/10.1038/sj.onc.1203716

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