Figure 5 | Oncogene

Figure 5

From: Inhibition of tumor growth and metastasis of human melanoma by intracellular anti-ATF-1 single chain Fv fragment

Figure 5

Intracellular ScFv anti-ATF-1 renders melanoma cells susceptible to apoptosis by thapsigargin (Tg). Apoptosis was assayed by propidium iodide staining and flow cytometry. (A) example of a representative analysis by flow cytometry of cells stained with propidium iodide after 48 h treatment with Tg. (B) a summary of at least three independent FACS analyses of propidium iodide staining following treatment with 1 μM Tg for 48 h. ScFv-transfected cells showed a higher rate of hypodiploid DNA contents indicative of apoptosis as compared with control cells. Apoptosis was also observed in KCREB-transfected cells

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