Figure 2 | Oncogene

Figure 2

From: Inhibition of tumor growth and metastasis of human melanoma by intracellular anti-ATF-1 single chain Fv fragment

Figure 2

Intracellular expression of ScFv anti-ATF-1 inhibits CRE-dependent promoter activation. Effect of ScFv anti-ATF-1 on CAT activity driven by a CRE-dependent promoter (Somat-BglII), was analysed in MeWo-P, Neo- or ScFv-transfected cells. CAT activity was inhibited by 10-fold and 35-fold in MeWo-ScFv-P and in MeWo-ScFv-C4, respectively (lanes 5 and 6) when compared to parental and Neo-transfected cells (lanes 2–4). The inhibition of CAT activity in ScFv-C4 was comparable to that observed in clone transfected with the dominant-negative construct of CREB, KCREB (35-fold, lane 7). Lane 1, Basic; control plasmid without the CRE-dependent promoter. -Fold inhibition is calculated in comparison with untransfected cells (lanes 2–4), which were given the reference value of 1. Figure is representative of three experiments performed

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