Figure 3 | Oncogene

Figure 3

From: Transformation of Madin-Darby canine kidney (MDCK) epithelial cells by Epstein-Barr virus latent membrane protein 1 (LMP1) induces expression of Ets1 and invasive growth

Figure 3

Enhanced motility of LMP1 transformants. (a) Invasion assay in 3-dimensional collagen gel. MDCK cells transfected with control plasmid (panel neo), and two clones of LMP1-transformed cells (panels: LMP1-2 and LMP1-3, respectively) were cultured in a 3-dimensional collagen gel matrix for 7 days. HGF (50 ng/ml) was added to the culture of MDCK cells transfected with control plasmid (panel: neo+HGF). Magnification=100×. (b) Scrape-wound migration assay. Confluent monolayers of MDCK cells transfected with control plasmid (panel: neo), and two clones of LMP1-transformed cells (panels: LMP1-2 and LMP1-3, respectively) were scraped by a plastic pipet, and wound-induced migration of cells was observed after 5 h. Magnification=150×

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