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The adenovirus E1A binding protein BS69 is a corepressor of transcription through recruitment of N-CoR

Oncogene volume 19, pages 1538–1546 (2000)Cite this article

Abstract

BS69 was first identified as a protein that interacts directly with the transactivation domain (conserved region 3) of the 289R adenovirus type 5 E1A protein. We show here that BS69 is a potent repressor of transcription. BS69 mediates repression, at least in part, through interaction with the co-repressor N-CoR. BS69 interacts with N-CoR through a MYND domain in its carboxyl terminus. A recently cloned splice variant of BS69, designated BRAM1, is also capable of interacting with N-CoR and E1A, but unlike BS69, is not able to repress transcription, indicating that N-CoR interaction is necessary but not sufficient for BS69 repression. Expression of E1A inhibits repression mediated by BS69. Our data suggest that BS69 participates in transcriptional repressor complexes and that E1A can modulate these complexes through interaction with BS69.

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Acknowledgements

We would like to thank Joseph Lipsick, Noma Collins, Achim Leutz and Stephane Ansieau for sharing unpublished data, Henk Stunnenberg for providing the FLAG-N-CoR cDNA, Marc Timmers for the luciferase reporter and Renate Zwijsen for advice on the experimental setup and helpful discussions. This work was supported by the Dutch Cancer Society and the Center for Biomedical Genetics.

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  1. Division of Molecular Carcinogenesis and Center for Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands

    Hans Masselink & René Bernards

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Masselink, H., Bernards, R. The adenovirus E1A binding protein BS69 is a corepressor of transcription through recruitment of N-CoR. Oncogene 19, 1538–1546 (2000). https://doi.org/10.1038/sj.onc.1203421

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