Acetyltransferases are essential enzymes for a wide variety of cellular processes and mutations in acetyltransferase genes have been associated with the development of certain cancers. For this reason, we conducted a computerized sequence homology search for novel acetyltransferases. Here, we show that the putative tumour suppressor protein Fus-2 has homology to the catalytic domain of acetyltransferases. We demonstrate that Fus-2 can acetylate the N-terminus of proteins using a ping-pong mechanism and that it has a specificity for substrates. Consistent with other N-acetyltransferases, Fus-2 localizes to the cytoplasm, as shown by GFP-tag experiments. Since the Fus-2 gene maps to the chromosomal region 3p21.3, which contains at least one tumour suppressor gene, the N-acetyltransferase functions of Fus-2 may be relevant to its potential role in cancer.
Your institute does not have access to this article
Subscribe to Journal
Get full journal access for 1 year
only $2.38 per issue
All prices are NET prices.
VAT will be added later in the checkout.
Tax calculation will be finalised during checkout.
Get time limited or full article access on ReadCube.
All prices are NET prices.
Bannister AJ and Kouzarides T . 1996 Nature 384: 641–643.
Bradshaw RA, Brickley WW and Walker KW . 1998 TIBS 23: 263–267.
Brauch H, Tory K, Kotler F, Gazdar AF, Pettengill OS, Johnson B, Graziano S, Winton T, Buys CH and Sorenson GD . 1990 Genes Chromosomes Cancer 1: 240–246.
Cascorbi I, Brockmoller J, Mrozikiewicz PM, Bauer S, Loddenkemper R and Roots I . 1996 Cancer Res 56: 3961–3966.
Dutnall RN, Tafrov ST, Sternglanz R and Ramakrishnan V . 1998 Cell 94: 427–438.
Grunstein M . 1997 Nature 389: 349–352.
Hibi K, Takahashi T, Yamakawa K, Ueda R, Sekido Y, Ariyoshi Y, Suyama M, Takagi H, Nakamura Y and Takahashi T . 1992 Oncogene 7: 445–449.
Johnson GG, Kronert WA, Bernstein SI, Chapman VM and Smith KD . 1988 J Biol Chem 263: 9079–9082.
Jornvall H, Fairwell T, Kratofil P and Wills C . 1980 FEBS Lett 111: 214–218.
Kok K, van den Berg A, Veldhuis PM, van der Veen AY, Franke M, Schoenmakers EF, Hulsbeek MM, van der Hout AH, de Leij L, van de Ven W and Buys CH . 1994 Cancer Res 54: 4183–4187.
Kouzarides T . 1999 Curr Opin Genet Dev 9: 40–48.
Mullen JR, Kayne PS, Moerschell RP, Tsunasawa S, Gribskov M, Colavito-Shepanski M, Grunstein M, Sherman F and Sternglanz R . 1989 EMBO J 8: 2067–2075.
Naylor SL, Johnson BE, Minna JD and Sakaguchi AY . 1987 Nature 329: 451–454.
Neuwald AF and Landsman D . 1997 Trends Biochem Sci 22: 154–155.
Wei MH, Latif F, Bader S, Kashuba V, Chen JY, Duh FM, Sekido Y, Lee CC, Geil L, Kuzmin I, Zabarovsky E, Klein G, Zbar B, Minna JD and Lerman MI . 1996 Cancer Res 56: 1487–1492.
Whang-Peng J, Kao-Shan CS, Lee EC, Bunn PA, Carney DN, Gazdar AF and Minna JD . 1982 Science 215: 181–182.
Wong LJ and Wong SS . 1983 Biochemistry 42: 4637–4641.
Zenser TV, Lakshmi VM, Rustan TD, Doll MA, Deitz AC, Davis BB and Hein DW . 1996 Cancer Res 56: 3941–3947.
We are grateful to Dr M Lerman for sending Cosmid LUCA-14 which contained the genomic Fus-2 DNA. We are also grateful to Nicole den Elzen and John Pines for supplying the GFP plasmids. This work was supported by grant (SP2081/0101) from the Cancer Research Campaign.
About this article
Cite this article
Zegerman, P., Bannister, A. & Kouzarides, T. The putative tumour suppressor Fus-2 is an N-acetyltransferase. Oncogene 19, 161–163 (2000). https://doi.org/10.1038/sj.onc.1203234
- tumour suppressor