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Distinction between AP1 and NF-E2 factor-binding at specific chromatin regions in mammalian cells

Oncogene volume 18, pages 5482–5486 (1999)Cite this article

Abstract

Specific nuclear factor-DNA complexes formed within the promoters and enhancers are essential for transcriptional regulation. For eukaryotic systems, however, some DNA motif(s) are capable of binding to a family of related factors, thus making it difficult to identify the factor actually binding on the chromatic DNA in vivo and modulating the local transcription processes. To resolve this matter, we have refined a chromatin immunoprecipitation assay. Using the assay, we could directly link the regulatory functions of two members of the AP1/NF-E2 transcription factor family and their stable binding in vivo within distinct chromatin regions. The study demonstrated the feasibility of a general scheme in the determination of the identity of specific factor(s), among a group of family members, bound at unique sequence(s) in living mammalian cells.

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Acknowledgements

We thank Xin Chen for helpful discussions, and Rama Gavva for the technical help. This research was supported by US Public Health grant (NIH DK 29800), and by the Academia Sinica, National Science Council, and National Health Research Institute, Taipei, Taiwan, ROC.

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Author notes

  1. Pratibha Daftari and Narender R Gavva: P Daftari and NR Gavva contributed equally to this work

Authors and Affiliations

  1. Section of Molecular and Cellular Biology, University of California, Davis, CA 95616, California, USA

    Pratibha Daftari, Narender R Gavva & C-K James Shen

  2. Institute of Molecular Biology, Academia Sinica, Nankang, Taipei, Taiwan

    C-K James Shen

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  1. Pratibha Daftari
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  2. Narender R Gavva
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Daftari, P., Gavva, N. & Shen, CK. Distinction between AP1 and NF-E2 factor-binding at specific chromatin regions in mammalian cells. Oncogene 18, 5482–5486 (1999). https://doi.org/10.1038/sj.onc.1202916

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