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Frequent loss of heterozygosity at 1p36 in ovarian adenocarcinomas but the gene encoding p73 is unlikely to be the target

Oncogene volume 18, pages 4640–4642 (1999)Cite this article

Abstract

Loss of heterozygosity (LOH) involving the distal part of the short arm of chromosome 1 occurs frequently in ovarian adenocarcinomas but the tumour suppressor gene(s) targeted by this event is unknown. We have used five microsatellite markers in a panel of 56 ovarian adenocarcinomas to determine which part of 1p34 – 36 is the focus of this LOH. LOH was considerably more common at 1p36 (43%) than at 1p34 – 35 (18%), and 11 tumours showed LOH at 1p36 but not at 1p34 – 35. These data strongly suggest the presence of a tumour suppressor gene inactivated in ovarian adenocarcinoma at 1p36. The p53 homologue, p73, has recently been isolated and mapped to 1p36 and therefore is a candidate for this tumour suppressor gene. However, RT – PCR and Western analyses revealed strong expression of p73 in ovarian adenocarcinoma cell lines but very low or undetectable levels in normal ovarian surface epithelial cells. Immunohistochemical analysis of primary ovarian tumours showed that only 3/22 (14%) contained p73 expressing cells. There was no association between 1p36 LOH and p73 expression in ovarian tumours, nor between p73 and p53 expression. These findings strongly suggest that p73 is not the target of 1p36 LOH in ovarian adenocarcinomas but indicate the presence of an, as yet unidentified, tumour suppressor gene in this region that plays an important role in ovarian tumorigenesis.

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Acknowledgements

This work was supported by the Australia/Russia Agreement in Medical Science, the National Health and Medical Research Council of Australia and Queensland Cancer Fund. We would like to thank S-K Khoo, B Ward and T Hurst for surgical specimens.

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Authors and Affiliations

  1. Group of Molecular Diagnostics, N.N. Petrov Institute of Oncology, St.-Petersburg, 189646, Russia

    Evgeny N Imyanitov

  2. The Queensland Institute of Medical Research, PO Royal Brisbane Hospital, Brisbane, 4029, Qld., Australia

    Geoff W Birrell, Jeremy Arnold, Michelle A Mould, Kim Wright, Martin F Lavin, Georgia Chenevix-Trench & Kum Kum Khanna

  3. Laboratory of Molecular Radiology Institute of Biophysics, Russian Federation Ministry of Health, Moscow, 123182, Russia

    Igor Filippovich & Natasha Sorokina

  4. Department of Pathology, The University of Queensland, PO Royal Brisbane Hospital, Brisbane, 4029, Qld., Australia

    Michael Walsh, Georgia Chenevix-Trench & Kum Kum Khanna

  5. Department of Obstetrics, Gynecology and Reproduction Biology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, Massachusetts, USA

    Samuel C Mok

  6. Department of Surgery, The University of Queensland, PO Royal Brisbane Hospital, Brisbane, 4029, Qld., Australia

    Martin F Lavin

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  1. Evgeny N Imyanitov
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Imyanitov, E., Birrell, G., Filippovich, I. et al. Frequent loss of heterozygosity at 1p36 in ovarian adenocarcinomas but the gene encoding p73 is unlikely to be the target. Oncogene 18, 4640–4642 (1999). https://doi.org/10.1038/sj.onc.1202863

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