Figure 8 | Oncogene

Figure 8

From: Inhibition of Bcl-xL expression sensitizes normal human keratinocytes and epithelial cells to apoptotic stimuli

Figure 8

Induction of apoptosis of hKn and A549 cells treated with cisplatinum (CP). (a) hKn cells were transfected with no oligonucleotide, 300 nM Bcl-xL antisense inhibitor (ISIS 16009), or 300 nM control (ISIS 20292) oligonucleotide. Twenty-four hours after transfection, cells were treated with the indicated level of cisplatinum (in μg/ml/10). Twenty-four hours after CP treatment, cells were harvested and analysed for apoptosis as described in the Materials and methods. (b) A549 cells were transfected with no oligonucleotide, 50 nM Bcl-xL inhibitor (ISIS 16009), or 50 nM control (ISIS 20292) oligonucleotide and treated with the indicated level of cisplatinum (in μg/ml). Twenty-four hours after CP treatment, cells were harvested as described above. The results shown are representative of three independent experiments

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