Figure 3 | Oncogene

Figure 3

From: Inhibition of Bcl-xL expression sensitizes normal human keratinocytes and epithelial cells to apoptotic stimuli

Figure 3

Inhibition of Bcl-xL expression by antisense oligonucleotides in hKn and A549 cells. (a) HKn cells were treated with Bcl-xL specific (ISIS 16009) or control (ISIS 20292) antisense oligonucleotides at the indicated concentrations and allowed to recover for 24 h. Bcl-xL and glyceraldehyde-3-phosphate dehydrogenase (G3PDH) mRNA levels were determined by Northern blot. (b) Bar graph of the quantitation of normalized Bcl-xL mRNA levels. Quantitation was performed by Phosphorimager analysis as described in Materials and methods. Percentage of control was calculated by comparison with Bcl-xL mRNA levels in cells that did not receive oligonucleotides. Results shown are from three independent experiments. (c) and (d) A549 cells were treated as above and the RNA was analysed as described in (a) and (b)

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