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Allelic loss mapping and physical delineation of a region harboring a putative thymic lymphoma suppressor gene on mouse chromosome 12

Oncogene volume 18, pages 4131–4136 (1999)Cite this article

Abstract

Our previous allelic loss analysis of γ-ray induced thymic lymphomas in F1 hybrid and backcross mice between BALB/c and MSM strains mapped the Tlsr4 region exhibiting a high frequency of allelic loss (62%) to a 2.9 cM interval between the markers D12Mit53 and D12Mit279 on mouse chromosome 12. To narrow further the interval harboring a putative tumor suppressor gene, a high-density scan has been carried out for informative 361 thymic lymphomas. Construction of a physical map of Tlsr4 with 3 YAC and 15 BAC clones and isolation of YAC- and BAC-derived polymorphic probes lead to fine allelic loss mapping. Three successive polymorphic sites within one BAC exhibit the retention of both alleles in seven, one and four lymphomas, suggesting that a common region of allelic loss for Tlsr4 exists within the BAC region. Pulsed-field gel electrophoresis of NotI digests of this and other clones determines that the commonly lost region is a 35 kb interval with a NotI site. NotI sites are frequently associated with coding regions, and our preliminary sequencing has identified ESTs in the region. Thus, the present study facilitates the identification of genes in the Tlsr4 region that would lead to isolation of a novel tumor suppressor gene.

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Abbreviations

YAC:

yeast artificial chromosome

BAC:

bacteria artificial chromosome

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Acknowledgements

This work was supported by Health Science Research Grants for Human Genome and Gene Therapy from Ministry of Health and Welfare of Japan.

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Authors and Affiliations

  1. Department of Biochemistry, Niigata University School of Medicine, Niigata, 951-8122, Asahimachi 1-757, Japan

    Toshimitsu Shinbo, Atsushi Matsuki, Yasuo Matsumoto, Shin-ichi Kosugi, Yoshiaki Takahashi & Ryo Kominami

  2. Department of Medicine (II), Niigata University School of Medicine, Asahimachi 1-757, Niigata, 951-8122, Japan

    Toshimitsu Shinbo

  3. Radiation Biology Center, Kyoto University, Yoshida-Konoecho, Sakyou-Ku, Kyoto, 606-8315, Japan

    Ohtsura Niwa

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  1. Toshimitsu Shinbo
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Shinbo, T., Matsuki, A., Matsumoto, Y. et al. Allelic loss mapping and physical delineation of a region harboring a putative thymic lymphoma suppressor gene on mouse chromosome 12. Oncogene 18, 4131–4136 (1999). https://doi.org/10.1038/sj.onc.1202767

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