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Differential expression assay of chromosome arm 8p genes identifies Frizzled-related (FRP1/FRZB) and Fibroblast Growth Factor Receptor 1 (FGFR1) as candidate breast cancer genes

Oncogene volume 18, pages 1903–1910 (1999)Cite this article

Abstract

Deletions and amplifications are frequent alterations of the short arm of chromosome 8 associated with various types of cancers, including breast cancers. This indicates the likely presence of tumor suppressor genes and oncogenes. In the present study, we have used the expressed sequence tag (EST) map of 8p11-21 to assemble a set of available cDNAs representing genes from this region. DNA arrays were prepared for expression analysis and search for genes potentially involved in breast cancer. Underexpresion in tumoral breast cells (versus normal breast) was observed for 15 transcripts. Among these, the Frizzled-related gene FRP1/FRZB, was turned off in 78% of breast carcinomas, suggesting that the lack of its product may be associated with malignant transformation. Overexpression in tumoral breast cells was observed for 13 genes. The FGFR1 gene, that encodes a tyrosine kinase receptor for members of the fibroblast growth factor family, was identified as a good candidate for one amplification unit. Taken together, our results demonstrate that such a strategy can rapidly identify genes with an altered pattern of expression and provide candidate genes for malignancies.

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Acknowledgements

We are grateful to Drs D Maraninchi, C Mawas, F Coulier, M Chaffanet, S Granjeaud, F Birg and P Pontarotti for help, discussions, and encouragement. This work was supported by INSERM, Institut Paoli-Calmettes, and grants from the Association pour la Recherche sur le Cancer, Ligue Nationale Contre le Cancer, and Ligue Nationale Contre le Cancer Comité des Bouches du Rhône. FU is recipient of MESR fellowship.

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Authors and Affiliations

  1. Laboratoire d'Oncologie Moléculaire, INSERM U119, 27 Bd Leï

    Françoise Ugolini, Daniel Birnbaum & Marie-Josèphe Pébusque

  2. Marseille, Roure, 13009, France

    Françoise Ugolini, Daniel Birnbaum & Marie-Josèphe Pébusque

  3. Laboratoire de Biologie des Tumeurs, Institut Paoli-Calmettes, Marseille, France

    José Adélaïde & Daniel Birnbaum

  4. Département d'Anatomo-Pathologie, Institut Paoli-Calmettes, Marseille, France

    Emmanuelle Charafe-Jauffret & Jocelyne Jacquemier

  5. TAGC/Centre d'Immunologie de Marseille Luminy, Marseille, France

    Catherine Nguyen, Bertrand Jordan & Daniel Birnbaum

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Ugolini, F., Adélaïde, J., Charafe-Jauffret, E. et al. Differential expression assay of chromosome arm 8p genes identifies Frizzled-related (FRP1/FRZB) and Fibroblast Growth Factor Receptor 1 (FGFR1) as candidate breast cancer genes. Oncogene 18, 1903–1910 (1999). https://doi.org/10.1038/sj.onc.1202739

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