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Identification and temporal expression pattern of genes modulated during irreversible growth arrest and terminal differentiation in human melanoma cells

Oncogene volume 18, pages 3546–3552 (1999)Cite this article

Abstract

Abnormalities in differentiation are common occurrences in human cancers. Treatment of human melanoma cells with the combination of recombinant human fibroblast interferon (IFN-β) and the antileukemic compound mezerein (MEZ) results in a loss of tumorigenic potential that correlates with an irreversible suppression in proliferative ability and induction of terminal differentiation. It is hypothesized that this is associated with the differential expression of genes that may directly regulate cancer cell growth and differentiation. To define the relevant gene expression changes that correlate with and potentially control these important cellular processes a differentiation induction subtraction hybridization (DISH) scheme is being used. A temporally spaced subtracted differentiation inducer treated (TSS) cDNA library was constructed and differentially expressed DISH clones were isolated and evaluated using a high throughput microchip cDNA (Synteni) array screening approach. Verification of differential gene expression for specific cDNAs was confirmed by Northern blotting. The temporal kinetics of regulation and the expression pattern of DISH genes were also evaluated by microchip cDNA array screening. Using this approach with 1000 DISH cDNA clones (∼10% of the DISH library) has resulted in the identification and cloning of both 26 known and 11 novel cDNAs of potential relevance to growth control and terminal differentiation in human melanoma cells.

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Acknowledgements

This research was supported in part by National Cancer Institute Grant CA35675, a sponsored research award from GenQuest Incorporated, the Samuel Waxman Cancer Foundation and the Chernow Research Endowment. PB Fisher is the Michael and Stella Chernow Urological Cancer Research Scientist in the Departments of Neurosurgery, Pathology and Urology.

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Authors and Affiliations

  1. GenQuest Incorporated, New York, 10032, NY, USA

    Fei Huang, Jennifer Adelman, Hongping Jiang & Neil I Goldstein

  2. Department of Neurosurgery, Herbert Irving Comprehensive Cancer Center, Columbia University, College of Physicians and Surgeons, New York, 10032, NY, USA

    Fei Huang & Paul B Fisher

  3. Department of Pathology, Herbert Irving Comprehensive Cancer Center, Columbia University, College of Physicians and Surgeons, New York, 10032, NY, USA

    Hongping Jiang & Paul B Fisher

  4. Department of Urology, Herbert Irving Comprehensive Cancer Center, Columbia University, College of Physicians and Surgeons, New York, 10032, NY, USA

    Fei Huang, Hongping Jiang & Paul B Fisher

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  1. Fei Huang
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  4. Neil I Goldstein
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Huang, F., Adelman, J., Jiang, H. et al. Identification and temporal expression pattern of genes modulated during irreversible growth arrest and terminal differentiation in human melanoma cells. Oncogene 18, 3546–3552 (1999). https://doi.org/10.1038/sj.onc.1202715

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