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HOX11 interacts with CTF1 and mediates hematopoietic precursor cell immortalization

Oncogene volume 18, pages 2273–2279 (1999)Cite this article

Abstract

HOX11 is a homeodomain-containing oncogenic transcription factor that immortalizes hematopoietic precursor cells. The mechanism by which HOX11 facilitates this initial step of leukemogenesis is, however, not well understood. We have used a DNA binding site selection assay to investigate cooperative DNA binding by HOX11 with other transcription factors. A consensus sequence was derived and identified as the binding site for the CCAAT-box-binding transcription factors (CTF). HOX11 was shown to interact in vitro and in vivo with CTF1. Retrovirus-mediated transduction of an antisense CTF1 cDNA dramatically reduced the proliferative capacity of HOX11-immortalized hematopoietic precursor cells. CTF1 is, therefore, the first HOX11 protein partner identified that plays an important role in hematopoietic precursor cell immortalization.

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Acknowledgements

We thank Dr C Largman for critical reading of the manuscript and Dr R Tjian for providing the CTF1 cDNA. Supported by grants from NIH (DK49619), National Leukemia Research Association and National Cancer Institute of Canada (No. 8398).

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Authors and Affiliations

  1. Cancer Center and Department of Medicine, University of California, San Diego, 9500 Gilman Drive, La Jolla, 92093-0671, California, USA

    Nan Zhang & Ming Lu

  2. Department of Medicine, University of California, San Francisco, 4150 Clement Street, San Francisco, 94121, California, USA

    Weifang Shen

  3. The Toronto Hospital and Department of Medical Biophysics, Oncology Gene Therapy Program, University of Toronto, Toronto, M5G 2M1, Ontario, Canada

    Robert G Hawley

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  1. Nan Zhang
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  2. Weifang Shen
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Zhang, N., Shen, W., Hawley, R. et al. HOX11 interacts with CTF1 and mediates hematopoietic precursor cell immortalization. Oncogene 18, 2273–2279 (1999). https://doi.org/10.1038/sj.onc.1202545

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