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Distinct chromosomal alterations associated with TP53 status of LoVo cells under PALA selective pressure: a parallel with cytogenetic pathways of colorectal cancers

Oncogene volume 18, pages 775–781 (1999)Cite this article

Abstract

This study investigates the chromosomal alterations involved in the acquisition of PALA resistance of LoVo colorectal cancer cells homozygous for wild-type TP53 before and after transfection with a 143Ala-mutated TP53 gene. PALA resistance was always associated with an increased number of CAD gene copies, but gene amplification sensu stricto was rarely observed. Interestingly, distinct chromosome patterns were found in relation to the TP53 status of the cells. In parental LoVo cells, the CAD copy number was increased through gains of normal chromosome 2 whereas in transfectant clones, resistance mostly occurred through chromosome rearrangements. The relationship with the two different cytogenetic patterns described in colorectal tumors is discussed.

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Acknowledgements

We are especially grateful to G Stark and B Vogelstein for generously providing the DNA constructs, to G Goubin, M Prosperi, J Villaudy, R Hamelin, A Nicolas, and E Lamoliatte for technical assistance. P Soulié has a fellowship from the Ministère de l'Enseignement Supérieur et de la Recherche.

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Authors and Affiliations

  1. Laboratoire de Cytogénétique Moléculaire et Oncologie, UMR 147 CNRS, Institut Curie, 26, rue d'Ulm, Paris, 75248, Cedex 05, France

    Priscilla Soulie, Marie-France Poupon, Yorghos Remvikos, Bernard Dutrillaux & Martine Muleris

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  1. Priscilla Soulie
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  2. Marie-France Poupon
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  3. Yorghos Remvikos
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Soulie, P., Poupon, MF., Remvikos, Y. et al. Distinct chromosomal alterations associated with TP53 status of LoVo cells under PALA selective pressure: a parallel with cytogenetic pathways of colorectal cancers. Oncogene 18, 775–781 (1999). https://doi.org/10.1038/sj.onc.1202336

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