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Interaction between Cdc37 and Cdk4 in human cells

Oncogene volume 14, pages 1999–2004 (1997)Cite this article

Abstract

Using the yeast two-hybrid system we have identified novel potential Cdk4 interacting proteins. Here we described the interaction of Cdk4 with a human homologue of the yeast Drosophila CDC37 gene products. Cdc37 protein specifically interacts with Cdk4 and Cdk6, but not with Cdc2, Cdk2, Cdk3, Cdk5 and any of a number of cyclins tested. Cdc37 is not an inhibitor nor an activator of the Cdk4/cyclin D1 kinase, while it appears to facilitate complex assembly between Cdk4 and cyclin D1 in vitro. Cdc37 competes with p16 for binding to Cdk4, suggesting that p16 might exert part of its inhibitory function by affecting the formation of Cdk4/cyclin D1 complexes via Cdc37.

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  1. Lou Lamphere and Francesca Fiore: The first two authors contributed equally to the work:

Affiliations

  1. Mitotix, Inc., One Kendall Square, Bldg. 660, Cambridge, 02139, Massachusetts, USA

    Lou Lamphere, Xu Xu, Leonardo Brizuela, Susan Keezer & Jeno Gyuris

  2. Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, 02139, Massachusetts, USA

    Claude Sardet

  3. Department of Experimental Oncology, European Institute of Oncology, 435 Via Ripamonti, Milan, 20141, Italy

    Francesca Fiore & Giulio F Draetta

Authors
  1. Lou Lamphere
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  2. Francesca Fiore
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  3. Xu Xu
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  4. Leonardo Brizuela
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  5. Susan Keezer
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  6. Claude Sardet
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  7. Giulio F Draetta
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  8. Jeno Gyuris
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Cite this article

Lamphere, L., Fiore, F., Xu, X. et al. Interaction between Cdc37 and Cdk4 in human cells. Oncogene 14, 1999–2004 (1997). https://doi.org/10.1038/sj.onc.1201036

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  • DOI: https://doi.org/10.1038/sj.onc.1201036

Keywords

  • Cdk
  • cyclin
  • cdc37
  • hsp90

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