Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Original Article
  • Published:

The 825C/T polymorphism of G-protein beta3 subunit gene and risk of ischaemic stroke

Journal of Human Hypertension volume 19, pages 709–714 (2005)Cite this article

Abstract

In this study, we investigated the association of G protein β3 subunit gene (GNB 3) C825T polymorphism with ischaemic stroke and its subtypes in the Chinese Han population in a large case–control study. A total of 990 ischemic stroke patients and 1124 controls were recruited from six medical centres in China. Genotype was determined by polymerase chain reaction and restriction fragment length polymorphism (RFLP) assay. Logistic regression analysis was performed to identify the independent risk factors for stroke. The frequency of 825T carriers is significantly higher in cerebral thrombosis in male subjects (OR=1.35, 95% CI, 1.01–1.82, P=0.046). After further adjustment with traditional risk factors to stroke, the association is not significant. In conclusion, the GNB3 825T allele is not an independent risk factor to ischaemic stroke in the Chinese population.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. He J, Klag MJ, Wu L, Whelton PK . Stroke in the People's Republic of China: II. Meta-analysis of hypertension and risk of stroke. Stroke 1995; 26: 2222–2227.

    Article  CAS  Google Scholar 

  2. World Health Organization. World Health Statistics Annual. 1993.

  3. Eastern Stroke and Coronary Heart Disease Collaborative Research Group. Blood pressure, cholesterol, and stroke in eastern Asia. Lancet 1998; 352: 1801–1807.

  4. Fernandez-Real JM, et al. G-protein β3 gene variant, vascular function, and insulin sensitivity in type 2 diabetes. Hypertension 2003; 41: 124–129.

    Article  CAS  Google Scholar 

  5. Siffert W et al. Association of a human G-protein beta3 subunit variant with hypertension. Nat Genet 1998; 18: 45–48.

    Article  CAS  Google Scholar 

  6. Siffert W . G-Protein β3 subunit 825T allele and hypertension. Curr Hypertens Rep 2003; 5: 47–53.

    Article  Google Scholar 

  7. Rosskopf D, Busch S, Manthey I, Siffert W . G-Protein b3 gene structure, promoter, and additional polymorphisms. Hypertension 2000; 36: 33–41.

    Article  CAS  Google Scholar 

  8. Huang X et al. Lack of association between the G-protein beta3 subunit gene and essential hypertension in Chinese: a case–control and a family-based study. J Mol Med 2003; 81: 729–735.

    Article  Google Scholar 

  9. Benson RT, Sacco RL . Stroke prevention: hypertension, diabetes, tobacco, and lipids. Neurol Clin 2000; 18: 309–319.

    Article  CAS  Google Scholar 

  10. Miller SA, Dykes DD, Polesky HF . A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 1998; 16: 1215.

    Article  Google Scholar 

  11. Sartori M et al. G-protein β3-subunit gene 825T allele and hypertension. Hypertension 2003; 42: 909–914.

    Article  CAS  Google Scholar 

  12. Poch E et al. G-Protein b3 subunit gene variant and left ventricular hypertrophy in essential hypertension. Hypertension 2000; 35: 214–218.

    Article  CAS  Google Scholar 

  13. Siffert W et al. Worldwide ethnic distribution of the G protein β3 subunit 825T allele and its association with obesity in Caucasians, Chinese, and black African individuals. J Am Soc Nephrol 1999; 10: 1921–1930.

    CAS  Google Scholar 

  14. Brand E, Wang J-G, Herrmann S-M, Staessen JA . An epidemiological study of blood pressure and metabolic phenotypes in relation to the Gβ3 C825T polymorphism. J Hypertens 2003; 21: 729–737.

    Article  CAS  Google Scholar 

  15. Morrison AC et al. G-Protein b3 subunit and a-adducin polymorphisms and risk of subclinical and clinical stroke. Stroke 2001; 32: 822–829.

    Article  CAS  Google Scholar 

  16. Cardon LR, Bell JI . Association study designs for complex diseases. Nat Rev Genet 2001; 2: 91–99.

    Article  CAS  Google Scholar 

  17. Yamamoto M et al. Association of GNB3 gene with pulse pressure and clustering of risk factors for cardiovascular disease in Japanese. Biochem Biophys Res Commun 2004; 316: 744–748.

    Article  CAS  Google Scholar 

  18. Speranza R et al. Atrial natriuretic peptide gene polymorphisms and risk of ischemic stroke in humans. Stroke 2004; 35: 814–818.

    Article  Google Scholar 

  19. Li S et al. Pentanucleotide TTTTA repeat polymorphism of apolipoprotein(a) gene and plasma lipoprotein(a) are associated with ischemic and hemorrhagic stroke in Chinese. Stroke 2003; 34: 1617–1622.

    Article  Google Scholar 

  20. Alessandro P et al. Synergistic effect of apolipoprotein E polymorphisms and cigarette smoking on risk of ischemic stroke in young adults. Stroke 2004; 35: 438–442.

    Article  Google Scholar 

  21. Alessandro P et al. Cumulative effect of predisposing genotypes and their interaction with modifiable factors on the risk of ischemic stroke in young adults. Stroke 2005; 36: 533–539.

    Article  Google Scholar 

  22. Andrea F et al. Proinflammatory genetic profiles in subjects with history of ischemic stroke. Stroke 2004; 35: 2270–2275.

    Article  Google Scholar 

  23. Amparo S et al. Homozygosity of the T allele of the 46 C → T polymorphism in the F12 gene is a risk factor for ischemic stroke in the spanish population. Stroke 2004; 35: 1795–1799.

    Article  Google Scholar 

  24. Martiskainen M et al. Fibrinogen gene promoter -455 A allele as a risk factor for lacunar stroke. Stroke 2003; 34: 886–891.

    Article  CAS  Google Scholar 

  25. Roberto P et al. Synergistic effect of -174 G/C polymorphism of the interleukin-6 gene promoter and 469 E/K polymorphism of the intercellular adhesion molecule-1 gene in Italian patients with history of ischemic stroke. Stroke 2003; 34: 881–885.

    Article  Google Scholar 

  26. Iacoviello L et al. Polymorphisms of the interleukin-1β gene affect the risk of myocardial infarction and ischemic stroke at young age and the response of mononuclear cells to stimulation in vitro. Arterioscler Thromb Vasc Biol 2005; 25: 222–227.

    Article  CAS  Google Scholar 

  27. Zhaohui L et al. Elevated plasma homocysteine was associated with hemorrhagic and ischemic stroke, but methylenetetrahydrofolate reductase gene C677T polymorphism was a risk factor for thrombotic stroke. Stroke 2003; 34: 2085–2090.

    Article  Google Scholar 

  28. Barbara V et al. Paraoxonase 192 Gln → Arg polymorphism: an independent risk factor for nonfatal arterial ischemic stroke among young adults. Stroke 2002; 33: 1459–1464.

    Article  Google Scholar 

  29. Jim J et al. Tissue plasminogen activator -7351C/T enhancer polymorphism is a risk factor for lacunar stroke. Stroke 2004; 35: 1090–1094.

    Article  Google Scholar 

Download references

Acknowledgements

This work was sponsored by the Ministry of Science and Technology of China (973 Project) and was supported by the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry.

Author information

Authors and Affiliations

  1. Sino-German Laboratory for Molecular Medicine, Fu Wai Hospital and Cardiovascular Institute, Chinese Academy of Medical Sciences, Beijing, People's Republic of China

    L Zhang, H Zhang, K Sun, Y Song, R Hui & X Huang

Authors
  1. L Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Y Song
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. R Hui
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. X Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to X Huang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, L., Zhang, H., Sun, K. et al. The 825C/T polymorphism of G-protein beta3 subunit gene and risk of ischaemic stroke. J Hum Hypertens 19, 709–714 (2005). https://doi.org/10.1038/sj.jhh.1001883

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.jhh.1001883

Keywords

This article is cited by

Search

Advanced search

Quick links