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GNB3 gene C825T and ACE gene I/D polymorphisms in essential hypertension in a Kazakh genetic isolate

Journal of Human Hypertension volume 18, pages 663–668 (2004)Cite this article

Abstract

The Kazakh inhabitants living in Barkol pasture of northeast China belong to a genetic isolate characterized by ethnically homogeneous and a communal pastoral lifestyle. To investigate whether the polymorphisms in the G-protein β-3 subunit (GNB3) gene and angiotensin-converting enzyme (ACE) gene are associated with essential hypertension (EH), we carried out a case–control study of 290 hypertensive subjects and 244 normotensive (NT) controls randomly selected from Kazakh populations of Barkol. A previous medical history of diabetes and hypertension, and body mass index (BMI) was recorded. Plasma glucose, triglyceride, and cholesterol were measured. The insertion/deletion (I/D) polymorphism of the ACE gene and the C825T polymorphism of the GNB3 gene were determined by the polymerase chain reaction (PCR) technique. The distributions of genotypes and alleles for the two polymorphisms did not differ significantly between the case and control populations, and odds ratio of EH related to the ACE gene D allele and GNB3 gene T allele was not significantly different from 1.0. Logistic regression analysis shows the variation at the GNB3 and ACE did not have any statistically significant synergistic effect on blood pressure (BP). Stratification of NT and untreated hypertensives according to I/D polymorphism of ACE gene and C825T polymorphism of GNB3 gene disclosed no significant difference across genotypes with respect to BMI, glucose, triglyceride, cholesterol, systolic and diastolic BP. In conclusion, the polymorphisms in the GNB3 gene and ACE gene, solely or combined, did not confer a significantly increased risk for the development of EH in the Kazakh isolate of northeast China.

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References

  1. Kurtz T, Spence M . Genetics of essential hypertension. Am J Med 1993; 94: 77–84.

    Article  CAS  PubMed  Google Scholar 

  2. Erdos EG . Angiotensin I-converting enzyme and the changes in our concepts through the years. Hypertension 1990; 16: 363–370.

    Article  CAS  PubMed  Google Scholar 

  3. Rigat B et al. PCR detection of the insertion/deletion polymorphism of the human angiotensin I converting enzyme gene (DCPI). Nucl Acids Res 1992; 20: 1433–1435.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Duru K et al. Frequency of a deletion polymorphism in the gene for angiotensin converting enzyme is increased in African-Americans with hypertension. Am J Hypertens 1994; 7: 759–762.

    Article  CAS  PubMed  Google Scholar 

  5. Zee R et al. Association of a polymorphism of the angiotensin I-converting enzyme gene with essential hypertension. Biochem Biophys Res Commun 1992; 184: 9–15.

    Article  CAS  PubMed  Google Scholar 

  6. Jeunemaitre X et al. Absence of human linkage between the angiotensinconverting enzyme locus and human essential hypertension. Nat Genet 1992; 1: 72–75.

    Article  CAS  PubMed  Google Scholar 

  7. Kiema T-R, Kauma H, Rantala AO, Lilja M . Variation at the angiotensin-converting enzyme gene and angiotensinogen gene loci in relation to blood pressure. Hypertension 1996; 28: 1070–1075.

    Article  CAS  PubMed  Google Scholar 

  8. Martinez E et al. Angiotensin-converting enzyme (ACE) gene polymorphisms, serum ACE activity and blood pressure in a Spanish-Mediterranean population. J Hum Hypertens 2000; 14: 131–135.

    Article  CAS  PubMed  Google Scholar 

  9. Bohm SK, Grady EF, Bunnet NW . Regulatory mechanisms that modulate signaling by G-protein-coupled receptors. Biochem J 1997; 322: 1–18.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Siffert W et al. Association of a human G-protein beta3 subunit variant with hypertension. Nat Genet 1998; 18: 45–48.

    Article  CAS  PubMed  Google Scholar 

  11. Beige J, Hohenbleicher H, Distler A, Sharma AM . G protein beta3 subunit C825T variant and ambulatory blood pressure in essential hypertension. Hypertension 1999; 33: 1049–1051.

    Article  CAS  PubMed  Google Scholar 

  12. Benjafield AV et al. G-protein β3 Subunit gene (GNB3) variant in causation of hypertension. Hypertension 1998; 32: 1094–1097.

    Article  CAS  PubMed  Google Scholar 

  13. Hegele R et al. G-protein β3-subunit gene variant and blood pressure variation in Canadian Oji-Cree. Hypertension 1998; 32: 688–692.

    Article  CAS  PubMed  Google Scholar 

  14. Larson N, Hutchinson R, Boerwinkle E . Lack of association of 3 functional gene variants with hypertension in African Americans. Hypertension 2000; 35: 1297–1300.

    Article  CAS  PubMed  Google Scholar 

  15. PRC National Blood Pressure Survey Cooperative Group. Prevalence and development trends of hypertension in China. Chin J Hypertens 1995; 3(Suppl): 7–13.

  16. Sambrook J, Fritsch EF, Maniatis T . Molecular Cloning: A Laboratory Manual, 2nd edn, Book 2. Cold Spring Harbor Laboratory Press: Cold Spring Harbur, NY, 1989, pp 456–469.

    Google Scholar 

  17. Olszanecka A et al. Ambulatory blood pressure and left ventricular structure and function inrelation to the G-protein β3 subunit polymorphism C825T in white Europens. J Hum Hypertens 2003; 17: 325–332.

    Article  CAS  PubMed  Google Scholar 

  18. Koto N et al. G-protein beta3 subunit gene and essential hypertension in Japanese. Hypertension 1998; 32: 688–692.

    Article  Google Scholar 

  19. Tsai CH et al. G-protein β3 subunit variant and essential hypertension in Taiwan—a case–control study. Int J Cardiol 2000; 73: 191–195.

    Article  CAS  PubMed  Google Scholar 

  20. Esther CR et al. The critical role of tissue angiotensin-converting enzyme as revealed by gene targeting in mice. J Clin Invest 1997; 99: 2375–2385.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Siffert W et al. Worldwide ethnic distribution of the G protein β3 subunit 825T allele and its association with obesity in Caucasian, Chinese, and Black African individuals. J Am Soc Nephrol 1999; 10: 1921–1930.

    CAS  PubMed  Google Scholar 

  22. O'Donnell CJ et al. Evidence for association and genetic linkage of the angiotensin-converting enzyme locus with hypertension and blood pressure in men but not women in the Framingham Heart Study. Circulation 1998; 97: 1766–1772.

    Article  CAS  PubMed  Google Scholar 

  23. Clark CJ et al. α-Adducin and angiotensin I–converting enzyme polymorphisms in essential hypertension. Hypertension 2000; 36: 990–994.

    Article  CAS  PubMed  Google Scholar 

  24. Rotimi C et al. Polymorphisms of rennin–angiotensin genes among Nigerians, Jamaicans, and African Americans. Hypertension 1996; 27: 558–563.

    Article  CAS  PubMed  Google Scholar 

  25. Kario K et al. Ischemic stroke and the gene for angiotensin-converting enzyme in Japanese hypertensives. Circulation 1996; 93: 1630–1633.

    Article  CAS  PubMed  Google Scholar 

  26. Rigat B et al. An insertion/deletion polymorphism in the angiotensin I-converting enzyme gene accounting for half the variance of serum enzyme levels. J Clin Invest 1990; 86: 1343–1346.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Tiret L et al. Evidence from combined segregation and linkage analysis, that a variant of the angiotensin I-converting enzyme (ACE) gene controls plasma ACE levels. Am J Hum Genet 1992; 51: 197–205.

    CAS  PubMed  PubMed Central  Google Scholar 

  28. Unger T et al. Angiotensin receptors. J Hypertens 1996; 14(Suppl): S95–S103.

    CAS  Google Scholar 

  29. Burke W et al. The Genetic Basis of Human Diseases. New York: Oxford University Press, New York, 1992; pp 170–191.

    Google Scholar 

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Acknowledgements

We thank Azati, Jinjia Liu Xiaoxian Xu, Nabei, and medical staff of Barkol Hospital for their help in our epidemiological survey. This work was supported by a grant from the Natural Science of China (No. 30160081).

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Authors and Affiliations

  1. Department of Cardiovasology, 1st Teaching Hospital, Xinjiang Medical University, Urumqi, 830054, PR, China

    X Wang, S Wang, Z Cheng & J Turdi

  2. Medical Research Center, 1st Teaching Hospital, Xinjiang Medical University, Urumqi, 830054, PR, China

    X Wang, R Lin, X Lu & H Wen

  3. Department of Neurobiology, Shanghai Second Medical University, Shanghai, 200025, PR, China

    X Jiang

  4. Department of Fundamental Medicine, Xinjiang Medical University, Urumqi, 830054, PR, China

    J Ding & G Wu

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  1. X Wang
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Correspondence to H Wen.

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Wang, X., Wang, S., Lin, R. et al. GNB3 gene C825T and ACE gene I/D polymorphisms in essential hypertension in a Kazakh genetic isolate. J Hum Hypertens 18, 663–668 (2004). https://doi.org/10.1038/sj.jhh.1001700

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