Mammalian tissues express at least two cannabinoid receptor types, CB1 and CB2, both G protein coupled. CB1 receptors are found predominantly at nerve terminals where they mediate inhibition of transmitter release. CB2 receptors occur mainly on immune cells, one of their roles being to modulate cytokine release. Endogenous agonists for cannabinoid receptors also exist, and are all eicosanoids. The first-discovered of these ‘endocannabinoids’ was arachidonoylethanolamide and there is convincing evidence that this ligand and some of its metabolites can activate vanilloid VRI (TRPV1) receptors. Certain cannabinoids also appear to have TRPV1-like and/or non-CB1, non-CB2, non-TRPV1 targets. Several CB1- and CB2-selective agonists and antagonists have been developed. Antagonists include the CB1-selective SR141716A, AM251, AM281 and LY320135, and the CB2-selective SR144528 and AM630. These all behave as inverse agonists, one indication that CB1 and CB2 receptors can exist in a constitutively active state. ‘Neutral’ cannabinoid receptor antagonists have also been developed. CB1 and/or CB2 receptor activation appears to ameliorate inflammatory and neuropathic pain and certain multiple sclerosis symptoms. This might be exploited clinically by using CB1, CB2 or CB1/CB2 agonists, or inhibitors of the membrane transport or catabolism of endocannabinoids that are released in increased amounts, at least in animal models of pain and multiple sclerosis. We have recently discovered the presence of an allosteric site on the CB1 receptor. Consequently, it may also prove possible to enhance ‘autoprotective’ effects of released endocannabinoids with CB1 allosteric enhancers or, indeed, to reduce proposed ‘autoimpairing’ effects of released endocannabinoids such as excessive food intake with CB1 allosteric antagonists.
Mammalian tissues are now known to express at least two types of cannabinoid receptor, both G-protein coupled.1 These are CB1 receptors, cloned in Tom Bonner's laboratory in 1990,2 and CB2 receptors, cloned by Sean Munro in 1993.3 This article provides an overview of the pharmacology of these receptors, where possible citing other review articles that provide more detailed information and list additional references.
Cannabinoid receptor signalling, distribution and functions
Both CB1 and CB2 receptors are coupled through Gi/o proteins, negatively to adenylate cyclase and positively to mitogen-activated protein kinase. CB1 receptors are also coupled through Gi/o proteins to certain ion channels, positively to inwardly rectifying and A-type outward potassium channels, and negatively to D-type outward potassium channels4 and to N-type and P/Q type calcium channels. CB1 receptors can also act through Gs proteins to activate adenylate cyclase. A more detailed description of these and other signalling mechanisms that have been proposed for cannabinoid CB1 and CB2 receptors can be found elsewhere.1
Although CB1 receptors are expressed by certain non-neuronal cells and tissues, for example the pituitary gland, immune cells and reproductive tissues, they are found predominantly at central and peripheral nerve terminals where they mediate inhibition of transmitter release.1 CB2 receptors occur mainly on immune cells, one of their roles being to modulate cytokine release. Thus, a common role of CB1 and CB2 receptors appears to be the modulation of ongoing release of chemical messengers, CB2 receptors from immune cells and CB1 receptors mainly from neurones.1 Interestingly, evidence is emerging that CB1 receptors can exist as homodimers and also that they may form heterodimers or oligomers with one or more other classes of coexpressed G protein-coupled receptor in a manner that may lead to cross-talk between CB1 and non-CB1 receptors.5 Indeed, it has been proposed that on-going cross-talk between presynaptic CB1 and α2-adrenergic receptors may account for the ability of clonidine to potentiate inhibition of electrically-evoked contractions of the mouse isolated vas deferens by the cannabinoid receptor agonist, R-(+)-WIN55212.6
The distribution pattern of CB1 receptors within the central nervous system accounts for several prominent effects of (–)-Δ9-tetrahydrocannabinol (Δ9-THC), the main psychotropic constituent of cannabis. Examples include its ability to decrease motor activity as indicated in rodents by hypokinesia and catalepsy, to induce signs of analgesia in animals and man and to stimulate food intake.1, 7, 8 Although often regarded as peripheral receptors, CB2 receptors have been detected in the central nervous system, for example on microglial cells.1, 5, 9
Endogenous agonists for cannabinoid receptors
The unequivocal demonstration that mammalian tissues express cannabinoid receptors was followed by the discovery that they can also produce endogenous ligands for these receptors.10 These ‘endocannabinoids’ are all eicosanoids, prominent examples including arachidonoylethanolamide (anandamide) and 2-arachidonoyl glycerol, both of which are synthesized on demand, removed from their sites of action by tissue uptake processes and metabolized mainly by fatty acid amide hydrolase (anandamide) or monoacylglycerol lipase (2-arachidonoyl glycerol).10 Endocannabinoids and their receptors constitute the ‘endocannabinoid system’.
It is likely that endocannabinoids function as both neuromodulators and immunomodulators and indeed, there is already evidence that within the central nervous system they serve as retrograde synaptic messengers.11 There is also evidence that there are some disease states or disorders in which endocannabinoids have an ‘autoprotective’ role. For example, results from animal experiments raise the possibility firstly, that increased amounts of endocannabinoid molecules may be released both in response to skeletal muscle spasm or spasticity in multiple sclerosis and in response to inflammatory pain, and secondly, that these released endocannabinoid molecules ameliorate such symptoms.8, 12 Thus, in addition to CB1 and CB2 receptors, other important pharmacological targets within the endocannabinoid system are the processes responsible for the biosynthesis, membrane transport and metabolism of endocannabinoids, as modulators of these processes clearly have therapeutic potential.
Cannabinoid receptor agonists
There are several established cannabinoid receptor agonists that bind more or less equally well to CB1 and CB2 receptors.1 The best known examples of these are
the ‘classical’ cannabinoids, Δ9-THC and (–)-11-hydroxy-Δ8-THC-dimethylheptyl (HU-210),
the ‘nonclassical’ cannabinoid, CP55940,
the aminoalkylindole cannabinoid, R-(+)-WIN55212, which has marginally greater CB2 than CB1 affinity, and
the eicosanoid cannabinoids, anandamide, which has marginally greater CB1 than CB2 affinity, and 2-arachidonoyl glycerol.
Of these, HU-210 has the highest affinity for both CB1 and CB2 receptors and HU-210, CP55940, R-(+)-WIN55212 and 2-arachidonoyl glycerol have the highest CB1 and CB2 relative intrinsic activities. Δ9-THC and anandamide have lower CB1 and CB2 affinities and relative intrinsic activities than these other cannabinoids. Indeed, at both receptor types, Δ9-THC and anandamide each exhibit the mixed agonist-antagonist properties that are typical of a partial agonist.1, 5 The recently discovered endocannabinoid, O-arachidonoylethanolamine (virodhamine), is also a CB1 receptor partial agonist and it too has been found to exhibit mixed agonist-antagonist properties at this receptor.13
A number of agonists with significant selectivity for CB1 or CB2 receptors have been developed.1, 5 Important CB1-selective agonists include the anandamide analogues, R-(+)-methanandamide, arachidonyl-2′-chloroethylamide (ACEA), arachidonyl-cyclopropylamide (ACPA) and O-1812. Of these both ACEA and ACPA share the susceptibility of anandamide to enzymic hydrolysis. In contrast, methanandamide and O-1812 are less susceptible to enzymic hydrolysis, probably because they are protected from this by the presence of a methyl substituent on the 1′ carbon. Another CB1-selective agonist of note is 2-arachidonyl glyceryl ether (noladin ether). The best CB2-selective agonists to have been developed so far include L-759633, L-759656 and JWH-133, all structural analogues of Δ9-THC, other notable examples being the nonclassical cannabinoid, HU-308, and the aminoalkylindole, AM1241.
Cannabinoid receptor agonists often contain chiral centres and these generally confer marked stereoselectivity in pharmacological assays. R-(+)-WIN55212 is more active than S-(–)-WIN55212 and classical and nonclassical cannabinoids with the same absolute stereochemistry as (–)-Δ9-THC at 6a and 10a (6aR, 10aR) have the greater activity. Anandamide itself does not contain any chiral centres. However, some of its synthetic analogues do, one example being methanandamide, the R-(+)-isomer of which has nine times greater affinity for CB1 receptors than the S-(–)-isomer.1
The discovery and pharmacological validation of new cannabinoid receptor agonists relies on the availability of suitable bioassays.1, 5 For CB1 receptor agonists, the most commonly used in vivo bioassay is the mouse tetrad, in which their ability to produce hypokinesia, hypothermia, catalepsy in the Pertwee ring test and antinociception in the tail-flick or hot plate test is determined in the same animal. There are no standard in vivo bioassays for CB2 receptor agonists. As to established in vitro bioassays for CB1 and CB2 receptor agonists, these all involve the use of membrane or tissue preparations that contain CB1 and/or CB2 receptors, expressed either naturally or after transfection.1, 5, 14 Among the most commonly used of these bioassays are binding assays that measure the ability of test compounds to displace a radiolabelled cannabinoid receptor ligand such as [3H]CP55940 from membranes obtained from CB1 and/or CB2 receptor-expressing cells or tissues. As to commonly used functional in vitro bioassays, some of these measure the effects of test compounds on CB1 or CB2 receptor signalling, for example stimulation of binding to G proteins of the hydrolysis-resistant GTP analogue [35S]GTPγS, Gi/o-mediated inhibition of basal or drug-induced cyclic AMP production and elevation of intracellular free Ca2+, which is presumably a Gs-mediated effect. The bioassay of CB1 receptor agonists can also be performed with isolated nerve-smooth muscle preparations such as the mouse vas deferens. These bioassays exploit the ability of cannabinoid agonists to act through neuronal CB1 receptors to produce a concentration-related inhibition of electrically-evoked contractile transmitter release and hence of the contractions resulting from this release. Strategies commonly used to validate effects as CB1 or CB2 receptor-mediated rely on the availability of selective CB1 and CB2 receptor antagonists (Cannabinoid CB1 and CB2 receptor antagonists), of cells or tissues that express either CB1 or CB2 receptors (but not both these receptor types), or of animals or tissues from which CB1 and/or CB2 receptors have been genetically deleted.
Repeated administration of cannabinoid CB1 receptor agonists can cause tolerance to develop to a number of their effects. This tolerance appears to be largely pharmacodynamic rather than pharmacokinetic in nature and to stem from CB1 receptor internalization and/or from a reduction in CB1 receptor protein synthesis or signalling.15 Similar mechanisms may underlie the development of tolerance to effects mediated by the CB2 receptor.16, 17
Cannabinoid CB1 and CB2 receptor antagonists
The discovery of CB1 and CB2 receptors was followed by the development of CB1- and CB2-selective cannabinoid receptor antagonists.1 Among these are the CB1-selective SR141716A, AM251, AM281 and LY320135. These all produce inverse cannabimimetic effects in at least some CB1 receptor-containing bioassay systems, effects that are opposite in direction from those produced by agonists for these receptors. Thus, for example, in vivo inverse effects of SR141716A in rats or mice include the production of signs of hyperalgesia in models of inflammatory and neuropathic pain, stimulation of intestinal motility and suppression of food consumption, while its in vitro inverse effects include enhancement of ongoing release of acetylcholine, noradrenaline and γ-aminobutyric acid in hippocampal slices, enhancement of the amplitude of electrically-evoked contractions of the mouse isolated vas deferens and inhibition of [35S]GTPγS binding to CB1 receptors in membrane preparations.18
There is evidence that not all of the inverse cannabimimetic effects of SR141716A, AM251, AM281 or LY320135 are produced through a single mechanism.18 Thus, it is likely that some of these inverse effects result from competitive surmountable antagonism at CB1 receptors of endogenously released endocannabinoids, a mechanism that is supported by evidence that such release takes place in a number of in vivo and in vitro bioassay systems. Some inverse cannabimimetic effects of ligands such as SR141716A are produced in the absence of any detectable ongoing endocannabinoid release. This is so both in systems into which CB1 receptors have been genetically inserted, and so are usually overexpressed, and in systems in which these receptors are expressed naturally. It is likely that some of these inverse effects are induced by a process of ‘inverse agonism’ in which CB1 receptors are shifted from a proposed constitutively active ‘on’ state to one or more constitutively inactive ‘off’ states. This putative mechanism relies on the assumption that CB1 receptors can exist in a constitutively active state in which they undergo some degree of spontaneous coupling to their effector mechanisms even in the absence of an endogenously released or exogenously added agonist. Support for this mechanism comes from the findings that it has proved possible, firstly to devise a CB1 mutant receptor at which SR141716A retains the ability to behave as an antagonist but loses its ability to produce an inverse cannabimimetic effect,19 and secondly to develop ‘neutral’ CB1 receptor antagonists. These ‘neutral’ antagonists share the ability of SR141716A to block responses to CB1 receptor agonists but lack its apparent ability to produce inverse cannabimimetic effects in CB1-containing systems in the absence of any endogenously released or exogenously added CB1 receptor agonist. Examples of ‘neutral’ CB1 receptor antagonists are 6″-azidohex-2″-yne-cannabidiol (O-2654), O-2050, a sulphonamide analogue of Δ8-THC with an acetylenic sidechain, and two SR141716A analogues, VCHSR and NESS 0327.18 Although, by definition, a ‘neutral’ antagonist cannot change the degree of any constitutive activity exhibited by CB1 receptors, it is expected to retain the ability to produce inverse cannabimimetic effects in tonically active biological systems when this tonic activity arises from ongoing endocannabinoid release onto CB1 receptors.
Not all inverse cannabimimetic effects of CB1 receptor ligands seem to be induced through CB1 receptor-dependent mechanisms. Thus, for example, there is evidence that at 10 μ M, SR141716A and AM251 inhibit [35S]GTPγS binding to rat cerebellar membranes by blocking the activation of A1 receptors by endogenously released adenosine.20 It has also been reported that at concentrations greater than 1 μ M SR141716A has a number of other actions that include inhibition of basal [35S]GTPγS binding to membranes obtained from CB1−/− mice and blockade of gap junctions and of certain types of ion channel.18, 21
SR141716A appears to be more potent at opposing effects induced by CB1 agonists than at producing inverse cannabimimetic effects by itself either at CB1 receptors or through CB1 receptor-independent mechanisms.18 It is possible, therefore, that there may be a low concentration range within which this ligand is essentially a neutral antagonist and that it is only at higher concentrations that it exhibits inverse agonist properties.
As to CB2-selective antagonists, the best known of these are SR144528 and AM630, both of which also produce inverse cannabimimetic effects in at least some cannabinoid receptor-containing bioassay systems.1 However, the mechanisms underlying the production of inverse effects by these two compounds have been little investigated.
The therapeutic potential of cannabinoid CB1 and CB2 receptor ligands
Dronabinol (Marinol), an oral preparation of Δ9-THC, and nabilone, a synthetic analogue of Δ9-THC are already licenced for clinical use in some countries as appetite stimulants (dronabinol) and antiemetics (both drugs). For CB1 receptor agonists, other potential uses include the management of glaucoma, pain, certain types of cancer and various kinds of motor dysfunction associated for example with multiple sclerosis or spinal cord injury.8, 12, 22, 23 Particularly convincing are preclinical, anecdotal and clinical data supporting the use of CB1 receptor agonists against inflammatory and neuropathic pain and for the amelioration of spasticity, muscle spasms, tremor or pain associated with multiple sclerosis or spinal cord injury.8, 12 Indeed, a Δ9-THC-containing extract of cannabis has now been developed in the UK as a medicine for the management of some multiple sclerosis symptoms. As to CB2 receptor agonists, there is already strong evidence that these have potential for the relief of inflammatory pain and, unexpectedly, also for the relief of neuropathic pain.24 While therapeutic targets for CB2 receptor inverse agonists/antagonists remain to be identified or validated, it is likely that the CB1 receptor inverse agonist/antagonist, SR141716A (Rimonabant/Acomplia), will soon be available in the clinic as an antiobesity agent.25, 26
Other pharmacological targets for cannabinoid receptor ligands
It is now clear that the TRPV1 (vanilloid VR1) receptor can be activated by anandamide, methanandamide and arachidonyl-2′-chloroethylamide (ACEA), although not by 2-arachidonoyl glycerol or by classical, nonclassical or aminoalkylindole cannabinoid receptor agonists (Cannabinoid receptor agonists) such as HU-210, CP55940 and R-(+)-WIN55212.5, 9, 27, 28 Evidence is also emerging for the existence of several other pharmacological targets that respond to at least some established cannabinoid receptor agonists or to abnormal-cannabidiol, a synthetic analogue of the plant cannabinoid cannabidiol that lacks significant affinity for both CB1 and CB2 receptors.5, 9 These include:
TRPV1-like receptors that are activated by WIN55212-2, CP55940 and capsaicin and that mediate inhibition of release of the excitatory neurotransmitter, glutamate, in brain areas such as the hippocampus;
various non-CB1, non-CB2, non-TRPV1 targets on central or peripheral neurones that modulate transmitter release when activated;
a non-neuronal target in mesenteric arteries that can be activated by abnormal-cannabidiol, methanandamide and anandamide but not Δ9-THC, HU-210, WIN55212-2 or 2-arachidonoyl glycerol to trigger reversal of α1-adrenoceptor-mediated vasoconstriction;
a target for abnormal-cannabidiol on microglial cells that can be activated to trigger migration of these cells towards neuroinflammatory lesion sites;
an SR144528-sensitive, SR141716A-insensitive, anandamide-insensitive, non-TRPV1, ‘CB2-like’ peripheral target through which palmitoylethanolamide, which lacks significant affinity for CB1 or CB2 receptors, can relieve inflammatory pain;
an allosteric site on the 5-HT3 receptor at which Δ9-THC can inhibit inward current through this ligand-gated cation channel with greater potency than cannabinoids such as R-(+)-WIN55212, anandamide, LY320135 and CP55940;
allosteric sites on GLUA1 and GLUA3 receptors, on M1 and M4 muscarinic receptors and on delayed rectifier potassium channels.
There is also evidence firstly, that palmitoylethanolamide can augment anandamide-induced microglial cell migration by acting through Gi/o-coupled receptors that are not CB1, CB2, ‘CB2-like’ or abnormal-cannabidiol receptors and secondly, that cannabinoids such as Δ9-THC and cannabidiol that contain a phenol group possess antioxidant (electron donor) activity that is sufficient to protect neurones against oxidative stress associated, for example, with glutamate-induced excitotoxicity.5
CB1 receptor antagonists/inverse agonists also appear to have pharmacological targets in addition to the CB1 receptor. For example, at concentrations above those at which it is capable of blocking the CB1 receptor, SR141716A behaves as an antagonist of the CB2 receptor, the putative abnormal-cannabidiol receptor, the adenosine A1 receptor, the TRPV1 receptor and possibly also the TRPV1-like receptor.18, 20
Finally, evidence is now emerging for the presence of an allosteric site on the CB1 receptor.29 Thus, we have found a series of novel compounds to behave as allosteric CB1 receptor modulators. These compounds do not displace [3H]CP55940 from CB1 binding sites but do modulate the rate at which [3H]CP55940 dissociates from these sites. This discovery opens up the possibility of developing allosteric CB1 antagonists instead of competitive CB1 receptor antagonists for the clinic (e.g. as antiobesity agents) and of developing allosteric CB1 enhancers that might be used therapeutically to augment the effects of endocannabinoids when these are released autoprotectively, for example in inflammatory pain conditions or in multiple sclerosis (Endogenous agonists for cannabinoid receptors).
Cannabinoid receptor research: future directions
A number of issues remain to be resolved. In particular, it will be important to establish
the extent to which proposed non-CB1, non-CB2 targets for CB1 or CB2 receptor agonists and antagonists/inverse agonists contribute to the pharmacology of these ligands, especially at clinically-relevant concentrations, or mediate physiological or pathological events of clinical importance;
whether it will prove possible to develop potent and selective non-CB1, non-CB2 agonists and antagonists for each of these proposed new targets;
the extent to which allosteric antagonism of the 5-HT3 receptor by Δ9-THC contributes towards the well-established antiemetic activity of this cannabinoid;
the mechanism by which CB2 receptors alleviate neuropathic pain if, as currently believed, CB2 receptors are not expressed by neurones;
whether the likely presence of an allosteric site on the CB1 receptor can be exploited in the clinic, for example, by employing an allosteric inhibitor as an anti-obesity agent or by using an allosteric enhancer for the management multiple sclerosis or chronic pain;
the extent to which cannabinoid receptors form CB1-CB1 and CB2-CB2 homodimers;
whether cannabinoid receptors form heterodimers or oligomers and, if so, the nature and extent of the resulting cross-talk between the endocannabinoid system and other endogenous systems.
Three other important issues that merit further investigation relate to the proposed ‘autoprotective’ role of endocannabinoids in some disease states (Endogenous agonists for cannabinoid receptors). The first of these concerns CB1 receptor antagonists/inverse agonists and neutral antagonists when these are used in the clinic, for example as appetite suppressants. Thus, the possibility arises that these drugs will enhance unwanted symptoms such as spasm, spasticity and pain if given to patients with multiple sclerosis or with disorders that cause inflammatory cause inflammatory pain in whom autoprotective release of endocannabinoids onto CB1 receptors is taking place.
The second of these issues concerns the potential use in the clinic of inhibitors of endocannabinoid membrane transport or of endocannabinoid enzymic hydrolysis by fatty acid amide hydrolase or monoacylglycerol lipase. Such inhibitors are likely to have fewer CB1 receptor-mediated side effects than directly-acting CB1 agonists as they are expected to augment CB1 receptor activation only in those parts of the endocannabinoid system in which endocannabinoid release is taking place. However, this does not necessarily mean that they will prove to have fewer or less marked unwanted side effects than directly-acting CB1 receptor agonists. For example, they may produce a new set of CB1 receptor-independent unwanted effects by inhibiting the enzymic inactivation of pharmacologically active endogenous molecules that do not serve as endocannabinoids or by causing an accumulation of endocannabinoid molecules at non-CB1, non-CB2 targets such as the TRPV1 receptor or the putative abnormal-cannabidiol receptor.5, 9 It is also to be expected that a fatty acid amide hydrolase inhibitor will change the pattern of anandamide metabolism so that there is a greater conversion of anandamide to pharmacologically active cyclooxygenase and/or lipoxygenase metabolites.11 The formation of cyclooxygenase metabolites will be even greater if there has also been induction of cyclooxygenase-2 by an inflammatory stimulus.
The third issue is whether CB1 receptor allosteric enhancers can be developed for the clinic. If they can, it is likely that they will prove to be even more selective than inhibitors of endocannabinoid membrane transport or enzymic hydrolysis in augmenting endocannabinoid-induced autoprotection to therapeutic advantage. Thus, like the transport and enzyme inhibitors, they are expected to increase CB1 receptor activation only at sites at which endocannabinoid release is taking place, thereby giving rise to fewer unwanted effects than directly-acting CB1 receptor agonists. However, unlike endocannabinoid transport and enzyme inhibitors they are not expected to augment the activation of non-CB1 targets by endocannabinoids, by endocannabinoid metabolites or by pharmacologically-active non-endocannabinoid substrates of endocannabinoid transporters or metabolizing enzymes.
Howlett AC, Barth F, Bonner TI, Cabral G, Casellas P, Devane WA et al. International Union of Pharmacology. XXVII. Classification of cannabinoid receptors. Pharmacol Rev 2002; 54: 161–202.
Matsuda LA, Lolait SJ, Brownstein MJ, Young AC, Bonner TT . Structure of a cannabinoid receptor and functional expression of the cloned cDNA. Nature 1990; 346: 561–564.
Munro S, Thomas KL, Abu-Shaar M . Molecular characterization of a peripheral receptor for cannabinoids. Nature 1993; 365: 61–65.
Mu J, Zhuang S-Y, Kirby MT, Hampson RE, Deadwyler SA . Cannabinoid receptors differentially modulate potassium A and D currents in hippocampal neurons in culture. J Pharmacol Exp Ther 1999; 291: 893–902.
Pertwee RG . Pharmacological actions of cannabinoids. In: Pertwee RG (ed) Cannabinoids. Handbook of Experimental Pharmacology. Springer-Verlag: Heidelberg, 2005, pp 1–51.
Pertwee RG, Thomas A, Stevenson LA, Maor Y, Mechoulam R . Evidence that (–)-7-hydroxy-4′-dimethylheptyl-cannabidiol activates a non-CB1, non-CB2, non-TRPV1 target in the mouse vas deferens. Neuropharmacology 2005; 48: 1139–1146.
Berry EM, Mechoulam R . Tetrahydrocannabinol and endocannabinoids in feeding and appetite. Pharmacol Ther 2002; 95: 185–190.
Pertwee RG . Cannabinoid receptors and pain. Prog Neurobiol 2001; 63: 569–611.
Pertwee RG . Novel pharmacological targets for cannabinoids. Curr Neuropharmacol 2004; 2: 9–29.
De Petrocellis L, Cascio MG, Di Marzo V . The endocannabinoid system: a general view and latest additions. Br J Pharmacol 2004; 141: 765–774.
Pertwee RG, Ross RA . Cannabinoid receptors and their ligands. Prostaglandins Leukot Essent Fatty Acids 2002; 66: 101–121.
Pertwee RG . Cannabinoids and multiple sclerosis. Pharmacol Ther 2002; 95: 165–174.
Porter AC, Sauer J-M, Knierman MD, Becker GW, Berna MJ, Bao J et al. Characterization of a novel endocannabinoid, virodhamine, with antagonist activity at the CB1 receptor. J Pharmacol Exp Ther 2002; 301: 1020–1024.
Pertwee RG . Pharmacology of cannabinoid CB1 and CB2 receptors. Pharmacol Ther 1997; 74: 129–180.
Sim-Selley LJ . Regulation of cannabinoid CB1 receptors in the central nervous system by chronic cannabinoids. Crit Rev Neurobiol 2003; 15: 91–119.
Massi P, Patrini G, Rubino T, Fuzio D, Parolaro D . Changes in rat spleen cannabinoid receptors after chronic CP-55,940: an autoradiographic study. Pharmacol Biochem Behav 1997; 58: 73–78.
Bouaboula M, Dussossoy D, Casellas P . Regulation of peripheral cannabinoid receptor CB2 phosphorylation by the inverse agonist SR 144528: implications for receptor biological responses. J Biol Chem 1999; 274: 20397–20405.
Pertwee RG . Inverse agonism and neutral antagonism at cannabinoid CB1 receptors. Life Sci 2005; 76: 1307–1324.
Pan X, Ikeda SR, Lewis DL . SR 141716A acts as an inverse agonist to increase neuronal voltage-dependent Ca2+ currents by reversal of tonic CB1 cannabinoid receptor activity. Mol Pharmacol 1998; 54: 1064–1072.
Savinainen JR, Saario SM, Niemi R, Järvinen T, Laitinen JT . An optimized approach to study endocannabinoid signaling: evidence against constitutive activity of rat brain adenosine A1 and cannabinoid CB1 receptors. Br J Pharmacol 2003; 140: 1451–1459.
Breivogel CS, Griffin G, Di Marzo V, Martin BR . Evidence for a new G protein-coupled cannabinoid receptor in mouse brain. Mol Pharmacol 2001; 60: 155–163.
Guzmán M . Cannabinoids: potential anticancer agents. Nat Rev Cancer 2003; 3: 745–755.
Tomida I, Pertwee RG, Azuara-Blanco A . Cannabinoids and glaucoma. Br J Ophthalmol 2004; 88: 708–713.
Malan TP, Ibrahim MM, Vanderah TW, Makriyannis A, Porreca F . Inhibition of pain responses by activation of CB2 cannabinoid receptors. Chem Phys Lipids 2002; 121: 191–200.
Van Gaal LF, Rissanen AM, Scheen AJ, Ziegler O, Rössner S . Effects of the cannabinoid-1 receptor blocker rimonabant on weight reduction and cardiovascular risk factors in overweight patients: 1-year experience from the RIO-Europe study. Lancet 2005; 365: 1389–1397.
Després J-P, Golay A, Sjöström L . Effects of rimonabant on metabolic risk factors in overweight patients with dyslipidemia. N Engl J Med 2005; 353: 2121–2134.
Price TJ, Patwardhan A, Akopian AN, Hargreaves KM, Flores CM . Modulation of trigeminal sensory neuron activity by the dual cannabinoid-vanilloid agonists anandamide, N-arachidonoyl-dopamine and arachidonyl-2-chloroethylamide. Br J Pharmacol 2004; 141: 1118–1130.
Baker CL, McDougall JJ . The cannabinomimetic arachidonyl-2-chloroethylamide (ACEA) acts on capsaicin-sensitive TRPV1 receptors but not cannabinoid receptors in rat joints. Br J Pharmacol 2004; 142: 1361–1367.
Price MR, Baillie GL, Thomas A, Stevenson LA, Easson M, Goodwin R et al. Allosteric modulation of the cannabinoid CB1 receptor. Mol Pharmacol 2005; 68: 1484–1495.
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Cite this article
Pertwee, R. The pharmacology of cannabinoid receptors and their ligands: an overview. Int J Obes 30, S13–S18 (2006). https://doi.org/10.1038/sj.ijo.0803272
- cannabinoid CB1 and CB2 receptors
- autoprotection and autoimpairment
- cannabinoid receptor inverse agonists and neutral antagonists
- allosteric site
- therapeutic targets and strategies
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