Abstract
Objective:
To evaluate the body fat distribution and fat cell size and number in an overweight/obese population from both genders, and to determine the possible relationship between fat cell data from three different adipose tissue localizations (subcutaneous (SA), perivisceral and omental) and adipose tissue composition and dietary fatty acid.
Design:
The sample consisted of 84 overweight/obese patients (29 men and 55 women) who have undergone abdominal surgery. The adipocyte size and total fat cell number was studied. Fat cell data were related with anthropometric, adipose tissue and subject's habitual diet fatty acid composition.
Measurements:
Fat cell size was measured according to a Sjöström method from the three adipose depots. Total fat cell number was also calculated. The fatty acid composition of adipose tissue was examined by gas chromatography. The subjects diet was studied by a 7 days dietary record.
Results:
Our data showed a negative relationship between the adipocyte size and the n-6 and n-3 fatty acids content of the SA adipose tissue (r=−0.286, P=0,040; r=−0.300, P=0.030) respectively, and the n-6 in the omental depots (r=−0.407, P=0.049) in the total population. Positive associations with the total of saturated (r=0.357, P=0.045) and negative (r=−0.544, P=0.001) with the n-9 fatty acids were observed when the relationship between the adipocyte number and the fatty acid composition of the different anatomical fat regions was studied. Dietary fatty acids composition positively correlated with fat cell size for the myristic acid (14:0) in men in the visceral depot (r=0.822, P=0.023), and for the saturated fatty acids (SFAs) in women in the omental depot (r=0.486, P=0.035).
Conclusion:
In the present study, for the first time in humans we found that n-3 and n-6 fatty acids are related to a reduced adipocyte size according to the depot localization. In contrast, adipose tissue and dietary SFAs sinificantly correlated with an increase in fat cell size and number. No significant associations were found between n-9 acids content and adipocyte size. However, n-9 adipose tissue fatty acids content was inversely associated with fat cell number showing that this type of fatty acid could limit hyperplasia in obese populations. The differences observed in the three different regions, perivisceral, omental and SA fat, indicate that this population adipose tissue have depot-specific differences.
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References
Duplus E, Glorian M, Forest C . Fatty acid regulation of gene transcription. J Biol Chem 2000; 275: 30749–30752.
Farnier C, Krief S, Blache M, Diot-Dupuy F, Mory G, Ferre P et al. Adipocyte functions are modulated by cell size change: potential involvement of an integrin/ERK signaling pathway. Int J Obes Relat Metab Disord 2003; 27: 1178–1186.
Le Soazig L, Krief S, Farnier C, Lefrere I, Le Liepvre X, Bazin R et al. Cholesterol, a cell-size dependent signal that regulates glucose metabolism and gene expression in adipocytes. J Biol Chem 2001; 276: 16904–16910.
Raclot T, Groscolas R, Langin D, Ferre P . Site-specific regulation of gene expression by n-3 polyunsaturated fatty acids in rat with adipose tissues. J Lipid Res 1997; 38: 1963–1972.
Pasquali R, Casimirri F, Morselli AM, Tortelli O, Pascal G, Anconetani B, et al., The VMH Collaborative Group. Body weight, fat distribution and the menopausal status in women. Int J Obes Relat Metab Disord 1994; 18: 614–621.
Durnin JVGA, Rahaman MM . The assessment of the amount of fat in the human body from skinfold thickness. Br J Nutr 1967; 21: 681–689.
Sjöström L . A computer tomography based multicompartment body composition technique and anthropometric prediction of lean body mass, total and subcutaneous adipose tissue. Int J Obes Relat Metab Disord 1991; 15: 19–30.
Tauri S, Tokunaga K, Fujioka S, Matsuzawa Y . Visceral fat obesity. Anthropological and patophysiological aspects. Int J Obes Relat Metab Disord 1991; 15: 1–8.
Sjöström L, Björntörp P, Vrana J . Microscopic fat cell size measurements on frozen-cut adipose tissue in comparison with automatic determination of osmium-fixed fat cells. J Lipid Res 1972; 12: 521–530.
Lepage G, Roy CC . Direct transesterification of all classes of lipids in a one-step reaction. J Lipid Res 1986; 27: 114–120.
Perez-llamas F, Garaulet M, Herrero F, Palma JT, Perez de Heredia F, Marín R et al. Multivalent informatics application for studies of the nutritional status of the population. Assessment of food intake. Nutrición Hospitalaria 2004; 19: 160–166 (in Spanish).
Mataix J, Mañas M, Llopis J, Martínez E [Tabla de composición de alimentos españoles]. Table of composition of Spanish foods. Instituto de Nutrición y Tecnología, Universidad de Granada: Granada, Spain, 1995. (in Spanish).
Azain MJ . Role of fatty acids in adipocyte growth and development. J Anim Sci 2004; 82: 916–924.
Garaulet M, Perez-Llamas F, Zamora S, Tebar FJ . Comparative study of the type of obesity in pre- and postmenopausal women: relationship with fat cell data, fatty acid composition and endocrine, metabolic, nutritional and psychological variables. Med Clin (Barc) 2002; 118: 281–286 (in Spanish).
Björntörp P . Adipocyte development. In: Björntörp P, Cairella M, Howard HA (eds) Proceedings from the Third International Congress on Obesity. Rome 1980. Libbey: London, 1981. pp 58–69.
Krotkiewski M, Sjöström L, Björntörp P, Smith U . Regional adipose tissue cellularity in relation to metabolism in young and middle-aged women. Metabolism 1975; 24: 703–710.
Hegsted DM, Jack CW, Stare FJ . The composition of human adipose tissue from several parts of the world. Am J Clin Nutr 1964; 14: 280–290.
Rodriguez VM, Pico C, Portillo MP, Macarulla MT, Palou A . fat source regulates ob gene expression in white adipose tissue of rats under hyperphagic feeding. Br J Nutr 2002; 87: 427–434.
Marin P, Andersson B, Ottosson M, Olbe L, Chowdhury B, Kvist H et al. morphology and metabolism of intraabdominal adipose tissue in men. Metabolism 1992; 41: 1242–1248.
Okuno M, Kajiwara K, Imai S, Kobayashi T, Honma T, Maki T et al. Perilla oil prevents the excessive growth of visceral adipose tissue in rats by down-regulating adipocyte differentiation. J Nutr 1997; 127: 1752–1757.
Belzung F, Raclot T, Groscolas R . Fish oil n-3 fatty acids selectively limit the hypertrophy of abdominal fat depots in growing rats fed high-fat diets. Am J Physiol 1993; 264: R1111–R1118.
Hill JO, Peters JC, Lin D, Yakubu F, Greene H, Swift L . Lipid accumulation and body fat distribution is influenced by type of dietary fat fed to rats. Int J Obes Relat Metab Disord 1993; 17: 223–236.
Fickova M, Hubert P, Cremel G, Leray C . Dietary (n-3) and (n-6) polyunsaturated fatty acids rapidly modify fatty acid composition and insulin effects in rat adipocytes. J Nutr 1998; 128: 512–519.
Sessler AM, Ntambi JM . Polyunsaturated fatty acid regulation of gene expression. J Nutr 1998; 128: 923–926.
Clandinin MT, Cheema S, Field CJ, Garg ML, Venkatraman J, Clandinin TR . Dietary fat: exogenous determination of membrane structure and cell function. FASEB J 1991; 5: 2761–2769.
Iacono JR, Dougherty RM . Effects of polyunsaturated fats on blood pressure. Annu Rev Nutr 1993; 13: 243–260.
Lands WEM . Biochemistry and physiology of n-3 fatty acids. FASEB J 1992; 6: 2530–2536.
Willumsen N, Skorve J, Hexeberg S, Rustan AC, Berge RK . The hypotriglyceridemic effect of eicosapentaenoic acid in rats is reflected in increased mithocondrial fatty acid oxidation followed by diminished lipogenesis. Lipids 1993; 28: 683–690.
Surette ME, Whelan J, Broughton KS, Kinsella JE . Evidence for mechanisms of hypotriglyceridemic effect of n-3 polyunsaturated fatty acids. Biochim Biophys Acta 1992; 1126: 199–205.
Amri EZ, Ailhaud G, Grimaldi PA . Fatty acids as signal transducing molecules: involvement in the differentiation of preadipose to adipose cells. J Lipid Res 1994; 35: 930–937.
Gaiva MHG, Couto RC, Oyama LM, Couto GEC, Silveira VLF, Riberio EB et al. Polyunsaturated fatty acid-rich diets: effect on adipose tissue metabolism in rats. Br J Med 2001; 86: 371–377.
Doucet E, Almeras N, White MD, Despres JP, Bouchard C, Tremblay A . Dietary fat composition and human adiposity. Eur J Clin Nutr 1998; 52: 2–6.
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Garaulet, M., Hernandez-Morante, J., Lujan, J. et al. Relationship between fat cell size and number and fatty acid composition in adipose tissue from different fat depots in overweight/obese humans. Int J Obes 30, 899–905 (2006). https://doi.org/10.1038/sj.ijo.0803219
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DOI: https://doi.org/10.1038/sj.ijo.0803219
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