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Changes in abdominal subcutaneous fat water content with rapid weight loss and long-term weight maintenance in abdominally obese men and women


OBJECTIVE: Insulin resistance decreases blood flow and volume in fat tissue. We hypothesised that fat tissue nutritive blood flow and volume, and thereby water content, would increase during weight loss and weight maintenance in obese persons.

DESIGN: Longitudinal clinical intervention with a 9-week very-low-calorie diet (VLCD) followed by one year of weight maintenance.

SUBJECTS: Obese men (n=13) and women (n=14) with the metabolic syndrome.

MEASUREMENTS: Water content of abdominal subcutaneous fat tissue as estimated by a sensor on the skin surface measuring the dielectric constant at 300 MHz. Anthropometric measures of fatness and fat distribution. Biochemical measures related to insulin resistance.

RESULTS: Subjects lost 14.5±3.4% of body weight during the VLCD, and generally sustained this weight loss during weight maintenance. Insulin sensitivity as estimated by an index (qualitative insulin sensitivity check index) increased during the VLCD, and remained increased throughout weight maintenance. The dielectric constant increased from 23.3±2.3 to 25.0±2.1 (P<0.001) during the VLCD, and further to 27.8±1.9 (P<0.001) during weight maintenance, indicating an increase in the water content of subcutaneous fat. The increase in subcutaneous fat water content did not correlate with weight loss and other measures of adiposity during the VLCD, but there was an inverse correlation that strengthened in significance from baseline to 6, 9 and 12 mo (r=−0.32 to −0.64, P=0.079–0.002). Increases in subcutaneous fat water content also correlated with improvements in insulin sensitivity at 6, 9 and 12 months of weight maintenance (r=0.34–0.54, P=0.094–0.006).

CONCLUSIONS: Water content of abdominal subcutaneous adipose tissue increases with weight loss in obese persons with the metabolic syndrome, and may reflect increased subcutaneous fat tissue nutritive blood flow. The increase in water content correlates with the increase in insulin sensitivity, suggesting that weight loss and consequent improved insulin sensitivity could mediate the increase in abdominal subcutaneous fat hydration.

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  1. Reaven GM . Banting lecture 1988. Role of insulin resistance in human disease. Diabetes 1988; 37: 1595–1607.

    CAS  Article  Google Scholar 

  2. Laakso M, Edelman SV, Brechtel G, Baron AD . Decreased effect of insulin to stimulate skeletal muscle blood flow in obese man. A novel mechanism for insulin resistance. J Clin Invest 1990; 85: 1844–1852.

    CAS  Article  Google Scholar 

  3. Blaak EE, van Baak MA, Kemerink GJ, Pakbiers MT, Heidendal GA, Saris WH . Beta-adrenergic stimulation and abdominal subcutaneous fat blood flow in lean obese, and, reduced-obese subjects. Metabolism 1995; 44: 183–187.

    CAS  Article  Google Scholar 

  4. Henry S, Schneiter P, Jequier E, Tappy L . Effects of hyperinsulinemia and hyperglycemia on lactate release and local blood flow in subcutaneous adipose tissue of healthy humans. J Clin Endocrinol Metab 1996; 81: 2891–2895.

    CAS  PubMed  Google Scholar 

  5. Laine H, Knuuti MJ, Ruotsalainen U, Raitakari M, Iida H, Kapanen J, Kirvela O, Haaparanta M, Yki-Jarvinen H, Nuutila P . Insulin resistance in essential hypertension is characterized by impaired insulin stimulation of blood flow in skeletal muscle. J Hypertens 1998; 16: 211–219.

    CAS  Article  Google Scholar 

  6. Fruhbeck G, Gomez-Ambrosi J, Muruzabal FJ, Burrell MA . The adipocyte: a model for integration of endocrine and metabolic signaling in energy metabolism regulation. Am J Physiol Endocrinol Metab 2001; 280: E827-E847.

    Article  Google Scholar 

  7. Larsen OA, Lassen NA, Quaade F . Blood flow through human adipose tissue determined with radioactive xenon. Acta Physiol Scand 1966; 66: 337–345.

    CAS  Article  Google Scholar 

  8. Jansson PA, Larsson A, Smith U, Lonnroth P . Glycerol production in subcutaneous adipose tissue in lean and obese humans. J Clin Invest 1992; 89: 1610–1617.

    CAS  Article  Google Scholar 

  9. Bolinder J, Kerckhoffs DA, Moberg E, Hagstrom-Toft E, Arner P . Rates of skeletal muscle and adipose tissue glycerol release in nonobese and obese subjects. Diabetes 2000; 49: 797–802.

    CAS  Article  Google Scholar 

  10. Mather K, Laakso M, Edelman S, Hook G, Baron A . Evidence for physiological coupling of insulin-mediated glucose metabolism and limb blood flow. Am J Physiol Endocrinol Metab 2000; 279: E1264–E1270.

    CAS  Article  Google Scholar 

  11. Clark MG, Colquhoun EQ, Rattigan S, Dora KA, Eldershaw TP, Hall JL, Ye J . Vascular and endocrine control of muscle metabolism. Am J Physiol 1995; 268: E797E812.

    Google Scholar 

  12. Raitakari M, Nuutila P, Knuuti J, Raitakari OT, Laine H, Ruotsalainen U, Kirvela O, Takala TO, Iida H, Yki-Jarvinen H . Effects of insulin on blood flow and volume in skeletal muscle of patients with IDDM: studies using [15O]H2O, [15O]CO, and positron emission tomography. Diabetes 1997; 46: 2017–2021.

    CAS  Article  Google Scholar 

  13. Laine H, Knuuti MJ, Ruotsalainen U, Utriainen T, Oikonen V, Raitakari M, Luotolahti M, Kirvela O, Vicini P, Cobelli C, Nuutila P, Yki-Jarvinen H . Preserved relative dispersion but blunted stimulation of mean flow, absolute dispersion, and blood volume by insulin in skeletal muscle of patients with essential hypertension. Circulation 1998; 97: 2146–2153.

    CAS  Article  Google Scholar 

  14. Bonadonna RC, Saccomani MP, Del Prato S, Bonora E, DeFronzo RA, Cobelli C . Role of tissue-specific blood flow and tissue recruitment in insulin-mediated glucose uptake of human skeletal muscle. Circulation 1998; 98: 234–241.

    CAS  Article  Google Scholar 

  15. Misra A, Garg A, Abate N, Peshock RM, Stray-Gundersen J, Grundy SM . Relationship of anterior and posterior subcutaneous abdominal fat to insulin sensitivity in nondiabetic men. Obes Res 1997; 5: 93–99.

    CAS  Article  Google Scholar 

  16. Abate N, Garg A, Peshock RM, Stray-Gundersen J, Grundy SM . Relationships of generalized and regional adiposity to insulin sensitivity in men. J Clin Invest 1995; 96: 88–98.

    CAS  Article  Google Scholar 

  17. World Health Organization. . Obesity. Preventing and managing the global epidemic. Report of a WHO consultation on obesity. WHO: Geneva, 3–5 June 1997.

  18. Barbe P, Stich V, Galitzky J, Kunesova M, Hainer V, Lafontan M, Berlan M . In vivo increase in beta-adrenergic lipolytic response in subcutaneous adipose tissue of obese subjects submitted to a hypocaloric diet. J Clin Endocrinol Metab 1997; 82: 63–69.

    CAS  PubMed  Google Scholar 

  19. Alanen E, Lahtinen T, Nuutinen J . Variational formulation of open-ended coaxial line in contact with layered biological medium. IEEE Trans Biomed Eng 1998; 45: 1241–1248.

    CAS  Article  Google Scholar 

  20. Smith SR, Foster KR . Dielectric properties of low-water-content tissues. Phys Med Biol 1985; 30: 965–973.

    CAS  Article  Google Scholar 

  21. Foster KR, Schwan HP . Dielectric properties of tissues and biological materials: a critical review. Crit Rev Biomed Eng 1989; 17: 25–104.

    CAS  PubMed  Google Scholar 

  22. Gabriel C, Gabriel S, Corthout E . The dielectric properties of biological tissues: I. Literature survey. Phys Med Biol 1996; 41: 2231–2249.

    CAS  Article  Google Scholar 

  23. Alanen E, Lahtinen T, Nuutinen J . Measurement of dielectric properties of subcutaneous fat with open-ended coaxial sensors. Phys Med Biol 1998; 43: 475–485.

    CAS  Article  Google Scholar 

  24. American Diabetes Association: Clinical Practice Recommendations 1997. Diabetes Care 1997; 20: S1–S70.

  25. Executive Summary of The. Third Report of The National Cholesterol Education Program (NCEP) Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults (Adult Treatment Panel III). JAMA 2001; 285: 2486–2497.

  26. Katz A, Nambi SS, Mather K, Baron AD, Follmann DA, Sullivan G, Quon MJ . Quantitative insulin sensitivity check index: a simple, accurate method for assessing insulin sensitivity in humans. J Clin Endocrinol Metab 2000; 85: 2402–2410.

    CAS  Article  Google Scholar 

  27. 1999 World Health Organization-International Society of Hypertension Guidelines for the Management of Hypertension. Guidelines Subcommittee. J Hypertens 1999; 17: 151–183.

  28. Maxwell MH, Heber D, Waks AU, Tuck ML . Role of insulin and norepinephrine in the hypertension of obesity. Am J Hypertens 1994; 7: 402–408.

    CAS  Article  Google Scholar 

  29. Sjostrom L, Rissanen A, Andersen T, Boldrin M, Golay A, Koppeschaar HP, Krempf M . Randomised placebo-controlled trial of orlistat for weight loss and prevention of weight regain in obese patients. European Multicentre Orlistat Study Group. Lancet 1998; 352: 167–172.

    CAS  Article  Google Scholar 

  30. Davidson MH, Hauptman J, DiGirolamo M, Foreyt JP, Halsted CH, Heber D, Heimburger DC, Lucas CP, Robbins DC, Chung J, Heymsfield SB . Weight control and risk factor reduction in obese subjects treated for 2 years with orlistat: a randomized controlled trial. JAMA 1999; 281: 235–242.

    CAS  Article  Google Scholar 

  31. Zhi J, Melia AT, Funk C, Viger-Chougnet A, Hopfgartner G, Lausecker B, Wang K, Fulton JS, Gabriel L, Mulligan TE . Metabolic profiles of minimally absorbed orlistat in obese/overweight volunteers. J Clin Pharmacol 1996; 36: 1006–1011.

    CAS  Article  Google Scholar 

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This study was financed in part by a research grant from Hoffmann-La Roche, Inc, (Baseal, Switzerland).

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Correspondence to D E Laaksonen.

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Laaksonen, D., Nuutinen, J., Lahtinen, T. et al. Changes in abdominal subcutaneous fat water content with rapid weight loss and long-term weight maintenance in abdominally obese men and women. Int J Obes 27, 677–683 (2003).

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  • Insulin resistance
  • Metabolic syndrome
  • Subcutaneous fat tissue
  • Very-low-calorie diet
  • Weight maintenance

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