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Genotype-by-smoking interaction for leptin levels in the Metabolic Risk Complications of Obesity Genes project

International Journal of Obesity volume 27, pages 334–340 (2003)Cite this article

Abstract

RATIONALE: Recently, we identified a genotype-by-smoking status interaction with serum leptin levels in a sample of Mexican Americans. However, it is unknown whether this phenomenon occurs in other populations as well.

OBJECTIVE: The goal of this study was to examine the genetic architecture of the response to smoking in leptin levels using data from Midwestern Caucasian subjects participating in the Metabolic Risk Complications of Obesity Genes project.

METHODS: We employed a variance decomposition analysis using maximum likelihood methods to model genotype-by-smoking interactions for leptin levels and examined the impact of the exclusion of smokers in a subsequent linkage analysis.

RESULTS: We found significant evidence (p-value=0.027) for a genotype-by-smoking status interaction for serum leptin levels. In the subsequent linkage analysis with smokers excluded, we obtained a maximum LOD score of 3.4 (P=0.00004) near D8S1128.

CONCLUSIONS: These results suggest that a QTL on chromosome 8 may have a differential effect on the expression of leptin in smokers vs nonsmokers, as first identified in Mexican Americans.

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Acknowledgements

This work was supported by grants HL34989, DK54026, MH59490, and RR00058 from the National Institutes of Health. The genotyping was undertaken through the auspices of the Mammalian Genotyping Service of the NIH, funds being allocated to the Marshfield Medical Research Foundation. TOPS, Inc. provided funds for the establishment of the families database, phenotyping, and linkage analysis. Part of the phenotyping costs was also provided through a collaborative research agreement with Millennium Pharmaceuticals, Inc.

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Authors and Affiliations

  1. Center for Epidemiology and Biostatistics and The Division of Human Genetics, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA

    L J Martin

  2. Department of Genetics, Southwest Foundation for Biomedical Research, San Antonio, TX, USA

    L J Martin, J Blangero & A G Comuzzie

  3. TOPS Center for Obesity and Metabolic Research, The Human and Molecular Genetics Center, Medical College of Wisconsin, Milwaukee, WI, USA

    A H Kissebah & G E Sonnenberg

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  1. L J Martin
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  2. A H Kissebah
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  3. G E Sonnenberg
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  4. J Blangero
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  5. A G Comuzzie
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Correspondence to L J Martin.

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Martin, L., Kissebah, A., Sonnenberg, G. et al. Genotype-by-smoking interaction for leptin levels in the Metabolic Risk Complications of Obesity Genes project. Int J Obes 27, 334–340 (2003). https://doi.org/10.1038/sj.ijo.0802232

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