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Effects of cafeteria diet feeding on β3-adrenoceptor expression and lipolytic activity in white adipose tissue of male and female rats

International Journal of Obesity volume 24, pages 1396–1404 (2000)Cite this article

Abstract

OBJECTIVE: To investigate the effects of short term (15 days) cafeteria diet feeding on the expression of β3-AR in vivo and its association with lipolytic stimulation induced by β3-AR agonist CGP12177A in isolated white adipocytes.

ANIMALS: Six female and 6 male Wistar rats (at 4 weeks of age) were fed on a cafeteria diet plus standard diet for 15 days. The remaining 12 age- and sex-matched rats always received standard diet only.

MEASUREMENTS: White gonadal adipose tissue was isolated and used for the determination of β3-AR and leptin expression, and for in vitro studies of lipolytic activity.

RESULTS: Control male rats had higher levels of both β3-AR and leptin mRNA in white adipose tissue than their female counterparts. Both male and female rats up-regulated the levels of both β3-AR and leptin mRNA in response to 15 day cafeteria diet feeding. Noradrenaline- and isoprenaline-induced lipolysis were significantly increased in fat cells from control females compared to their male counterparts. CGP12177A stimulation resulted in significantly higher glycerol release in fat cells from cafeteria diet-fed female rats, whereas there were no differences due to dietary treatment in male rats. The maximal lipolytic response of forskolin (stimulating adenylyl cyclase) and dibutyryl cyclic AMP (cyclic AMP analogous) was not affected by sex or cafeteria diet feeding.

CONCLUSION: Cafeteria diet feeding brings about higher excess body weight and impaired adipose tissue lipolytic activity in female rats compared to male rats. Thus, the higher levels of β3-AR mRNA induced by cafeteria feeding are not indicative per se of an increase of the lipolytic response of the adipocytes. The changes seen in other adrenoceptor subtypes (β1 and β2) may be more determinant of the overall lipolytic response of adipocytes.

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Acknowledgements

This work was supported by Dirección General de Enseñanza Superior e Investigación Científica of the Spanish Government (grant PM97-0094) and the European Commission (contract No. ERBCHRX© CT940490 and COST Action 918).

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  1. Laboratori de Biologia Molecular, Nutrició i Biotecnologia, Departament de Biologia Fonamental i Ciències de la Salut, Universitat de les Illes Balears, Palma de Mallorca, Spain

    I Lladó, ME Estrany, E Rodríguez, B Amengual, P Roca & A Palou

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Correspondence to A Palou.

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Lladó, I., Estrany, M., Rodríguez, E. et al. Effects of cafeteria diet feeding on β3-adrenoceptor expression and lipolytic activity in white adipose tissue of male and female rats. Int J Obes 24, 1396–1404 (2000). https://doi.org/10.1038/sj.ijo.0801390

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