Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Anti-obesity effects of lipase inhibitor CT-II, an extract from edible herbs, Nomame Herba, on rats fed a high-fat diet

International Journal of Obesity volume 24, pages 758–764 (2000)Cite this article

Abstract

OBJECTIVE: To investigate the inhibitory effects of CT-II, extract of Nomame Herba, on lipase activity in vitro and on obesity in rats fed a high-fat diet in vivo.

DESIGN: The assay for the inhibitory effect of CT-II on lipase activity was performed by measuring released free fatty acids after the incubation of the medium with CT-II, porcine pancreatic lipase and triolein (experiment 1). In vivo experiments, lean rats or obese rats (570–718 g) were fed a high-fat diet containing 60% fat with or without CT-II for 8 weeks (experiment 2), for 14 days (experiment 3) or for 12 weeks (experiment 4), respectively.

MEASUREMENT: The time course of body weight, food intake, organ weight (parametrial fat, liver, heart and kidney) and plasma parameters (triglyceride, total cholesterol, glucose, AST, ALT and insulin), fecal output of total fat and total cholesterol were measured. Hepatic histological examinations were also performed.

RESULTS: CT-II inhibited the porcine lipase activity dose-dependently in vitro (experiment 1). Body and liver weight were reduced and hepatic histological examination showed an amelioration of fatty liver in CT II treated animals (experiment 2). CT-II significantly inhibited body weight gain and plasma triglyceride elevation in a dose-dependent manner, without affecting food intake in lean rats fed the high-fat diet. Elevated plasma AST and ALT were also decreased (experiment 3). When obese rats fed the high-fat diet were treated with CT-II for up to 6 months, body weight was initially reduced and thereafter weight gain was significantly suppressed. Total body fat was also significantly reduced and significant reduction of plasma AST and ALT was observed (experiment 4).

CONCLUSIONS: These results demonstrated that the lipase inhibitor CT-II is effective in preventing and ameliorating obesity, fatty liver and hypertriglyceridemia in rats fed a high-fat diet.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Get just this article for as long as you need it

$39.95

Learn more

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2
Figure 3
Figure 4

References

  1. Borgstöm B . Luminal digestion of fats. In Go L (ed.) The exocrine pancreas: biology, pathobiology and diseases Raven Press: New York 1986 pp 361–373.

    Google Scholar 

  2. Hadváy P, Lengsfeld H, Wolfer H . Inhibition of pancreatic lipase in vitro by covalent inhibitor tetrahydrolipstatin Biochem J 1988 256: 357–361.

    Article  Google Scholar 

  3. Drent ML, Larsson I, William-Olsson T, Quaade F, Czubayko F, Von Bergmann K, Strobel W, Sjöström L, Van der Veen EA . Orlistat (RO 18-0647), a lipase inhibitor, in the treatment of human obesity: a multiple dose study Int J Obes Relat Metab Disord 1995 19: 221–226.

    CAS  PubMed  Google Scholar 

  4. Hauptman JB, Jeunet FS, Hartmann D . Initial studies in humans with the novel gastrointestinal lipase inhibitor Ro 18-0647 (tetrahydrolipstatin) Am J Clin Nutr 1992 55: 309S–313S.

    Article  CAS  PubMed  Google Scholar 

  5. Sjöström L, Rissanen A, Andersen T, Boldrin M, Golay A, Koppeschaar HPF . Randomised placebo-controlled trial of orlistat for weight loss and prevention of weight regain in obese patients Lancet 1998 352: 167–172.

    Article  PubMed  Google Scholar 

  6. Lairon D, Borel P, Termine E, Grataroli R, Chabert C, Hauton JC . Evidence for a proteinic inhibitor of pancreatic lipase in cereals, wheat bran and wheat germ Nutr Rep Int 1985 32: 1107–1113.

    CAS  Google Scholar 

  7. Garugouri Y, Julien R, Pieroni G, Verger R, Sarda L . Studies on the inhibition of pancreatic and microbial lipases by soybean proteins J Lipid Res 1984 25: 1214–1221.

    Google Scholar 

  8. Satouchi K, Matsushita S . Purification and properties of a lipase inhibiting protein from soybean cotyledons Agric Biol Chem 1976 40: 889–897.

    CAS  Google Scholar 

  9. Shimura S, Tsuzuki W, Kobayashi S, Suzuki T . Inhibitory effect on lipase activity of extracts from medicinal herbs Biosci Biotechnol Biochem 1992 56: 1478–1479.

    Article  CAS  Google Scholar 

  10. Fukumoto J, Iwai M, Tsujisaka Y. Studies on lipase I . Purification and crystallization of a lipase secreted by Aspergillus niger J Gen Appl Microbiol 1963 9: 353–361.

    Article  Google Scholar 

  11. Inoue S, Campfield LA, Bray GA . Comparison of metabolic alterations in hypothalamic and high fat diet-induced obesity Am J Physiol 1977 233 (3): R162–R168.

    CAS  PubMed  Google Scholar 

  12. Morgan C R, Lazarow A . Immunoassay of insulin: two antibody system. Plasma insulin levels in normal, subdiabetic and diabetic rats Diabetes 1963 12: 115–126.

    Article  Google Scholar 

  13. Folch J, Lees M, Sloane-Stanley GA . A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 1957 226: 497–509.

    CAS  PubMed  Google Scholar 

  14. Borgström B . Mode of action of tetrahydrolipstatin: A derivative of the naturally occurring lipase inhibitor lipstatin Biochim Biophys Acta 1988 962: 308–316.

    Article  PubMed  Google Scholar 

  15. Fernandez E, Borgström B . Effect of tetrahydrolipstatin, a lipase inhibitor, on absorption of fat from the intestine of the rat Biochim Biophys Acta 1989 1001: 249–255.

    Article  CAS  PubMed  Google Scholar 

  16. Hogan S, Fleury A, Hadváy P, Lengsfeld H, Meier MK, Triscari J, Sullivan AC . Studies on antiobesity activity of tetrahydrolipstatin, a potent and selective inhibitor of pancreatic lipase Int J Obes 1987 11 (Suppl 3): 35–42.

    CAS  PubMed  Google Scholar 

  17. Hatano T, Yamashita A, Hashimoto T, Ito H, Kubo N, Yoshiyama M, Shimura S, Itoh Y, Okuda T, Yoshida T . Flavan dimers with lipase inhibitory activity from Cassia nomame Phytochemistry 1997 46: 893–900.

    Article  CAS  Google Scholar 

  18. Bernfeld P. In Colwich SB, Kaplan NO (ed) . Methods of enzymology. Academic Press: New York 1955, pp 149–150.

    Book  Google Scholar 

  19. Shimura S, Tsuzuki W, Itoh Y, Kobayashi S, Suzuki T . Inhibitory effect of tannin fraction from Cassia mimosoides L. var. nomame Makino on lipase activity Nippon Shokuhin Kogyo Gakkaishi 1994 41: 561–564.

    Article  CAS  Google Scholar 

  20. Zamora R, Hidalgo FJ, Tappel AL . Comparative antioxidant effectiveness of dietary β-carotene, vitamin E, selenium and coenzyme Q10 in rat erythrocytes and plasma J Nutr 1991 121: 50–56.

    Article  CAS  PubMed  Google Scholar 

  21. Nakano T, Kanmuri T, Sato M, Takeuchi M . Effect of astaxanthin rich red yeast (Phaffia rhodozyma) on oxidative stress in rainbow trout Biochim Biophys Acta 1999 1426: 119–125.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biotechnology, Lotte Co. Ltd, 3-1-1 Numakage, Urawa-shi, Saitama, Japan

    M Yamamoto, S Shimura & Y Itoh

  2. Division of Geriatric Health and Nutrition, National Institute of Health and Nutrition, 1-23-1 Toyama, Shinjyuku-ku, Tokyo, Japan

    T Ohsaka & S Inoue

  3. Division of Pathophysiology, College of Nursing, Yokohama City University, 3-9 Fukuura, Kanazawa-ku, Yokohama-shi, Kanagawa, Japan

    M Egawa

Authors
  1. M Yamamoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S Shimura
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Y Itoh
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. T Ohsaka
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M Egawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. S Inoue
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Yamamoto, M., Shimura, S., Itoh, Y. et al. Anti-obesity effects of lipase inhibitor CT-II, an extract from edible herbs, Nomame Herba, on rats fed a high-fat diet. Int J Obes 24, 758–764 (2000). https://doi.org/10.1038/sj.ijo.0801222

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.ijo.0801222

Keywords

  • Cassia mimosoides
  • lipase inhibitor
  • obesity
  • fatty liver
  • hypertriglyceridemia

This article is cited by

Search

Advanced search

Quick links