Volume 42 Issue 6, June 2021

Supplementation of chitosan oligosaccharides(COS) could ameliorate severity of pancreatic injury, prevent intestinal barrier disruption and reduce inflammatory and oxidative injury in severe acute pancreatitis (SAP). The protective mechanism partly attributed to the reshaping of gut microbiota and TLR4 and Nrf2/HO-1 pathway.

Upon LPS stimulation, macrophages undergo metabolic reprogramming to enhance glycolysis with suppressed oxidative metabolism, which further increased succinate accumulation and SDH activity to boost inflammatory response. Tan-IIA remarkably inhibited mitochondrial ROS generation through interrupting SDH hyperactivation to further reduce HIF-1α stabilization and transcription. And for that, attenuated glycolysis and preserved Sirt2 activity contributed to suppression of NLRP3 inflammatory activation to further reduce the maturation of IL-1β.