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| Open AccessCloning of the wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoides
Leaf rust is one of the most severe foliar diseases of wheat. Here, the authors report the cloning of Lr47, a broadly effective leaf rust resistance gene introgressed into wheat from Aegilops speltoides, and show it encodes a coiled-coil nucleotide-binding leucine-rich repeat protein.
- Hongna Li
- , Lei Hua
- & Shisheng Chen
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Article
| Open AccessCloning of the broadly effective wheat leaf rust resistance gene Lr42 transferred from Aegilops tauschii
The Aegilops tauschii-derived leaf rust resistance gene Lr42 has been widely used for breeding resistance wheat cultivars, but the molecular basis is unknown. Here, the authors show that Lr42 encodes an NLR-type of disease resistance gene by bulked segregant mapping in Ae. tauschii and confirm its function in common wheat.
- Guifang Lin
- , Hui Chen
- & Sanzhen Liu
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| Open AccessChromosome evolution and the genetic basis of agronomically important traits in greater yam
While greater yam provides food and income security for millions of people around the world, there are limited genomic resources available. Here, the authors report a chromosome-scale assembly of the greater yam genome as well as quantitative trait loci associated with anthracnose resistance and tuber traits.
- Jessen V. Bredeson
- , Jessica B. Lyons
- & Daniel S. Rokhsar
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Article
| Open AccessThe interplay of additivity, dominance, and epistasis on fitness in a diploid yeast cross
Heritable traits can be affected by additive, dominance, and epistatic effects at genetic loci. Here, the authors use chromosomally-encoded barcodes to perform linkage mapping in diploid cross progeny in budding yeast, finding that epistasis in diploids frequently modifies both additivity and dominance.
- Takeshi Matsui
- , Martin N. Mullis
- & Ian M. Ehrenreich
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Article
| Open AccessAn ancestral NB-LRR with duplicated 3′UTRs confers stripe rust resistance in wheat and barley
Stripe rust is a serious threat to wheat production. Here, the authors reveal that the resistance gene, only present in the wheat progenitor Aegilops tauschii and its derived synthetic wheat, encodes a nucleotide oligomerization domain-like receptor and confers resistance in common wheat and barley.
- Chaozhong Zhang
- , Lin Huang
- & Daolin Fu
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Article
| Open AccessFast genetic mapping of complex traits in C. elegans using millions of individuals in bulk
QTL mapping in multicellular animals usually requires generation, maintenance, and phenotyping large panels of recombinant individuals. Here, the authors develop ceX-QTL mapping, a method for fast genetic mapping via bulk selection on millions of unique recombinant C. elegans individuals.
- Alejandro Burga
- , Eyal Ben-David
- & Leonid Kruglyak
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Article
| Open AccessThe complex underpinnings of genetic background effects
Mutations often show distinct phenotypic effects across different genetic backgrounds. Here the authors describe the genetic basis of these so-called background effects using data on genotype and growth in 10 environments from 1411 segregants from a cross of two strains of budding yeast.
- Martin N. Mullis
- , Takeshi Matsui
- & Ian M. Ehrenreich
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Article
| Open AccessProbability of phenotypically detectable protein damage by ENU-induced mutations in the Mutagenetix database
Programs such as PolyPhen-2 predict the relative severity of damage by missense mutations. Here, Wang et al estimate probabilities that putative null or missense alleles would reduce protein function to cause detectable phenotype by analyzing data from ENU-induced mouse mutations.
- Tao Wang
- , Chun Hui Bu
- & Bruce Beutler
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Article
| Open AccessPowerful decomposition of complex traits in a diploid model
Dissecting the architecture of complex trait is challenging. Here, Hallin, Märtens et al. devises Phased Outbred Lines (POLs) in order to accurately decompose growth trait variation in diploid yeast across different environments.
- Johan Hallin
- , Kaspar Märtens
- & Gianni Liti
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Article
| Open AccessGenetic lineage tracing defines myofibroblast origin and function in the injured heart
The origin and fate of myofibroblasts, the cells responsible for cardiac remodelling and fibrosis, is controversial. Here the authors show that cardiac myofibroblasts express periostin, derive exclusively from tissue-resident fibroblasts, are necessary for scar formation after injury, and can revert back to a less-activated state upon injury resolution.
- Onur Kanisicak
- , Hadi Khalil
- & Jeffery D. Molkentin