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This protocol presents ProBac sequencing, a bacterial single-cell RNA sequencing methodology using droplet microfluidics and large oligonucleotide probe sets.
Untargeted mass spectrometry (MS) produces complex, multidimensional data. The MZmine open-source project enables processing of spectral data from various MS platforms, e.g., liquid chromatography–MS, gas chromatography–MS, MS–imaging and ion mobility spectrometry–MS, and is specialized for metabolomics.
Community-generated online templates for harmonized data reporting ensure that data and metadata associated with experiments are findable, accessible, interoperable, reusable and compiled for consistency in experimental design and test performance.
This protocol is for using xMarkerFinder, a four-stage computational framework, to enable the identification and validation of reproducible microbial biomarkers from cross-cohort studies, and establish potential microbiome-induced mechanisms.
The Genome Editing Collection has recently been updated to feature a selection of Protocols, Reviews, Research and News articles published across the Nature Journals in the last two years.
The 52nd European Environmental Mutagenesis and Genomics Society (EEMGS) meeting and 15th International Comet Assay Workshop (ICAW) will be held in Rovinj, Croatia, from September 23rd to 27th, 2024. Deadlines are coming up this month for early registration and abstract submission.
Our authors are invited to write blog posts that describe how they conceived and developed their protocols, prior to publication at Nature Protocols. These stories are published on a community website for researchers who are interested in techniques and methods.
This Collection highlights articles on how to improve methodological clarity in science publications from a spectrum of different journals and subject areas.
Untargeted mass spectrometry (MS) produces complex, multidimensional data. The MZmine open-source project enables processing of spectral data from various MS platforms, e.g., liquid chromatography–MS, gas chromatography–MS, MS–imaging and ion mobility spectrometry–MS, and is specialized for metabolomics.
This protocol presents ProBac sequencing, a bacterial single-cell RNA sequencing methodology using droplet microfluidics and large oligonucleotide probe sets.
MSstats is used for the statistical analysis of proteomics data from DIA mass spectrometry experiments. This protocol describes workflows using (i) the MSstatsShiny graphical user interface and (ii) the R packages MSstats or MSstatsBig (for larger datasets).
This tutorial provides guidelines on oncolytic virus design, production and testing in cancer immunotherapy. Best practice recommendations for preclinical and clinical use of oncolytic viruses as an immunotherapy tool and related future challenges are also considered.
Springer Nature recently acquired protocols.io, an open-access platform for developing and sharing protocols, which will replace the Protocol Exchange from June 2024.
Community-generated online templates for harmonized data reporting ensure that data and metadata associated with experiments are findable, accessible, interoperable, reusable and compiled for consistency in experimental design and test performance.
This protocol is for using xMarkerFinder, a four-stage computational framework, to enable the identification and validation of reproducible microbial biomarkers from cross-cohort studies, and establish potential microbiome-induced mechanisms.
This protocol presents a metabolomics method tailored for detecting and measuring gut-microbe-derived metabolites using a broad reference library of metabolite standards.
This protocol describes toxin activation–inhibition conjugation (TAC–TIC), a reverse genetics screening approach that can be used to identify triggers or blockers of bacterial toxin–antitoxin or phage immunity systems.