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Signals from the reproductive system regulate the lifespan of C. elegans

Nature volume 399, pages 362–366 (1999)Cite this article

Abstract

Understanding how the ageing process is regulated is a fascinating and fundamental problem in biology. Here we demonstrate that signals from the reproductive system influence the lifespan of the nematode Caenorhabditis elegans. If the cells that give rise to the germ line are killed with a laser microbeam, the lifespan of the animal is extended. Our findings suggest that germline signals act by modulating the activity of an insulin/IGF-1 (insulin-like growth factor) pathway that is known to regulate the ageing of this organism. Mutants with reduced activity of the insulin/IGF-1-receptor homologue DAF-2 have been shown to live twice as long as normal1,2,3, and their longevity requires the activity of DAF-16, a member of the forkhead/winged-helix family of transcriptional regulators1,2,4,5. We find that, in order for germline ablation to extend lifespan, DAF-16 is required, as well as a putative nuclear hormone receptor, DAF-12 (refs 6, 7). In addition, our findings suggest that signals from the somatic gonad also influence ageing, and that this effect requires DAF-2 activity. Together, our findings imply that the C. elegans insulin/IGF-1 system integrates multiple signals to define the animal's rate of ageing. This study demonstrates an inherent relationship between the reproductive state of this animal and its lifespan, and may have implications for the co-evolution of reproductive capability and longevity.

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Figure 1: Response of wild-type nematodes to ablation of germline and somatic-gonad precursors.
Figure 2: Responses of daf-16 and daf-12 mutants to ablation of germline andsomatic-gonad precursors.
Figure 3: Responses of daf-2 mutants to ablation of the germline and somatic-gonad precursors.
Figure 4: A model for the effects of reproductive signals on lifespan.

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Acknowledgements

We thank J. Apfeld, K. Lin, R. Kindt, Q. Ch'ng, J. Whangbo, J. Alcedo, J. Dorman, B.Albinder, N. Oliviera, A. Dillon, D. Crawford, D. Garigan and other members of the Kenyon laboratory for discussions and comments on the manuscript. We also thank M. de Bono for providing the wild C.elegans strains and J. Maloof for advice on ablation procedures. This study was supported by an NIH grant to C.K.

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  1. Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, 94143-0448, California, USA

    Honor Hsin & Cynthia Kenyon

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  1. Honor Hsin
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  2. Cynthia Kenyon
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Correspondence to Cynthia Kenyon.

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Hsin, H., Kenyon, C. Signals from the reproductive system regulate the lifespan of C. elegans. Nature 399, 362–366 (1999). https://doi.org/10.1038/20694

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