Original Article
The Pharmacogenomics Journal (2003) 3, 41–52. doi:10.1038/sj.tpj.6500147
Analysis of gene expression in carbon tetrachloride-treated rat livers using a novel bioarray technology
M B Young1, M R DiSilvestro2, T J Sendera1,3, J Freund2, A Kriete2 and S R Magnuson1
- 1Motorola Life Sciences, 4088 Commercial Avenue, Northbrook, IL, USA
- 2TissueInformatics, Inc., Suite 202 Pittsburgh, PA, USA
- 3Amersham Biosciences, 3200 West Germann Rd., Chandler, 72 85248 USA
Correspondence: TJ Sendera, Amersham Biosciences, 3200 West Germann Rd., Chandler, 72 85248 USA, 480 413 3607. E-mail: timothy-sendera@Amersham biosciences.com
Received 18 March 2002; Revised 25 October 2002; Accepted 29 October 2002.
Abstract
The present study successfully utilizes a new ADME Rat Expression Bioarray, containing 1040 metabolism- and toxicology-linked genes, to monitor gene expression from the livers of rats treated with carbon tetrachloride (CCl4). Histopathological analysis, hierarchical clustering methods, and gene expression profiling are compared between the control and CCl4-treated animals. A total of 44 transcripts were found to be altered in response to the hepatotoxin, 19 of which were upregulated and 25 were downregulated. Some of these gene expression changes were expected and concurred with previously published data while others were novel findings.
Keywords:
hepatotoxicity, toxicogenomics, microarray, biochip, DNA chip, carbon tetrachloride
