Original Article

Citation: Translational Psychiatry (2013) 3, e218; doi:10.1038/tp.2012.130
Published online 22 January 2013

Differential gene body methylation and reduced expression of cell adhesion and neurotransmitter receptor genes in adverse maternal environment
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J-e Oh1, N Chambwe2, S Klein1, J Gal3, S Andrews2, G Gleason1, R Shaknovich4, A Melnick4, F Campagne2 and M Toth1

  1. 1Department of Pharmacology, Weill Cornell Medical College, New York, NY, USA
  2. 2Department of Physiology and Biophysics and HRH Prince Alwaleed Bin Talal Bin Abdulaziz Alsaud Institute for Computational Biomedicine, Weill Cornell Medical College, New York, NY, USA
  3. 3Harold and Margaret Milliken Hatch Laboratory of Neuroendocrinology, The Rockefeller University, New York, NY, USA
  4. 4Department of Medicine, Weill Cornell Medical College, New York, NY, USA

Correspondence: Dr J-e Oh or Dr M Toth, Department of Pharmacology, Weill Cornell Medical College, New York, NY 10065, USA. E-mail: jio2002@med.cornell.edu or mtoth@med.cornell.edu

Received 25 July 2012; Revised 12 September 2012; Accepted 6 October 2012

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Abstract

Early life adversity, including adverse gestational and postpartum maternal environment, is a contributing factor in the development of autism, attention deficit hyperactivity disorder (ADHD), anxiety and depression but little is known about the underlying molecular mechanism. In a model of gestational maternal adversity that leads to innate anxiety, increased stress reactivity and impaired vocal communication in the offspring, we asked if a specific DNA methylation signature is associated with the emergence of the behavioral phenotype. Genome-wide DNA methylation analyses identified 2.3% of CpGs as differentially methylated (that is, differentially methylated sites, DMSs) by the adverse environment in ventral-hippocampal granule cells, neurons that can be linked to the anxiety phenotype. DMSs were typically clustered and these clusters were preferentially located at gene bodies. Although CpGs are typically either highly methylated or unmethylated, DMSs had an intermediate (20–80%) methylation level that may contribute to their sensitivity to environmental adversity. The adverse maternal environment resulted in either hyper or hypomethylation at DMSs. Clusters of DMSs were enriched in genes that encode cell adhesion molecules and neurotransmitter receptors; some of which were also downregulated, indicating multiple functional deficits at the synapse in adversity. Pharmacological and genetic evidence links many of these genes to anxiety.

Keywords:

CpG island; development; DNA methylation; exon; maternal effect