X-ray crystallography

Definition

X-ray crystallography is a technique that uses X-ray diffraction patterns to determine high-resolution, three-dimensional structures of molecules such as proteins, small organic molecules, and materials. The substance of interest must be in crystalline form, which typically requires testing various crystallization conditions.

Latest Research and Reviews

  • Research | | open

    Neutralizing antibodies to oligomannose glycans on HIV Env are difficult to elicit, possibly due to B cell tolerance. Here, Pantophlet et al. synthesize mimetics based on a bacterial oligosaccharide and show that they evoke HIV-neutralizing antibody responses in animals with a human Ig repertoire.

    • Ralph Pantophlet
    • , Nino Trattnig
    • , Sasha Murrell
    • , Naiomi Lu
    • , Dennis Chau
    • , Caitlin Rempel
    • , Ian A. Wilson
    •  & Paul Kosma
  • Research | | open

    Using photosensitive caged-compound for femtosecond crystallography at X-ray free electron lasers would allow the structure determination of reaction intermediates. Here the authors demonstrate the feasibility of this approach with a caged NO-compound and present the initial NO-bound intermediate structure of cytochrome P450 nitric oxide reductase.

    • Takehiko Tosha
    • , Takashi Nomura
    • , Takuma Nishida
    • , Naoya Saeki
    • , Kouta Okubayashi
    • , Raika Yamagiwa
    • , Michihiro Sugahara
    • , Takanori Nakane
    • , Keitaro Yamashita
    • , Kunio Hirata
    • , Go Ueno
    • , Tetsunari Kimura
    • , Tamao Hisano
    • , Kazumasa Muramoto
    • , Hitomi Sawai
    • , Hanae Takeda
    • , Eiichi Mizohata
    • , Ayumi Yamashita
    • , Yusuke Kanematsu
    • , Yu Takano
    • , Eriko Nango
    • , Rie Tanaka
    • , Osamu Nureki
    • , Osami Shoji
    • , Yuka Ikemoto
    • , Hironori Murakami
    • , Shigeki Owada
    • , Kensuke Tono
    • , Makina Yabashi
    • , Masaki Yamamoto
    • , Hideo Ago
    • , So Iwata
    • , Hiroshi Sugimoto
    • , Yoshitsugu Shiro
    •  & Minoru Kubo
  • Research | | open

    Plasmodium falciparum protein Pfs25 is a promising malaria transmission blocking vaccine antigen. Here, Scally et al. determine the crystal structure of Pfs25 and identify antigenic sites that are recognized by transmission-blocking antibodies elicited in human immunoglobulin loci transgenic mice.

    • Stephen W. Scally
    • , Brandon McLeod
    • , Alexandre Bosch
    • , Kazutoyo Miura
    • , Qi Liang
    • , Sean Carroll
    • , Sini Reponen
    • , Ngan Nguyen
    • , Eldar Giladi
    • , Sebastian Rämisch
    • , Vidadi Yusibov
    • , Allan Bradley
    • , Franck Lemiale
    • , William R. Schief
    • , Daniel Emerling
    • , Paul Kellam
    • , C. Richter King
    •  & Jean-Philippe Julien
  • Research | | open

    Legionella pneumophila replicates in a Legionella-containing vacuole (LCV). Here the authors present the structure of the Legionella effector RidL N-terminal domain and reveal how RidL contributes to the subversion of retrograde trafficking by binding to the retromer coat complex subunit Vps29, which leads to a displacement of the regulator TBC1D5.

    • Kevin Bärlocher
    • , Cedric A. J. Hutter
    • , A. Leoni Swart
    • , Bernhard Steiner
    • , Amanda Welin
    • , Michael Hohl
    • , François Letourneur
    • , Markus A. Seeger
    •  & Hubert Hilbi
  • Research | | open

    An efficient vaccine for human metapneumovirus (hMPV) will likely rely on neutralizing antibodies against the fusion protein (F). Here, the authors determine the crystal structure of pre-fusion-stabilized hMPV F and identify a dense glycan shield that affects generation of neutralizing antibodies.

    • Michael B. Battles
    • , Vicente Más
    • , Eduardo Olmedillas
    • , Olga Cano
    • , Mónica Vázquez
    • , Laura Rodríguez
    • , José A. Melero
    •  & Jason S. McLellan

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