X-ray crystallography

X-ray crystallography is a technique that uses X-ray diffraction patterns to determine high-resolution, three-dimensional structures of molecules such as proteins, small organic molecules, and materials. The substance of interest must be in crystalline form, which typically requires testing various crystallization conditions.

Latest Research and Reviews

  • Research | | open

    Orthobunyaviruses (OBVs) cause severe disease in humans and farm animals, but the molecular basis for infection is not fully understood. Here, the authors present crystal structures of free and antibody-bound OBV envelope glycoproteins and show that their domains enable efficient immunization in a mouse model.

    • Jan Hellert
    • , Andrea Aebischer
    • , Kerstin Wernike
    • , Ahmed Haouz
    • , Emiliana Brocchi
    • , Sven Reiche
    • , Pablo Guardado-Calvo
    • , Martin Beer
    •  & Félix A. Rey
  • Research |

    A bacterial family of cGAS/DncV-like nucleotidyltransferases synthesizes a diverse range of cyclic dinucleotide and trinucleotide compounds that are likely to modulate the interaction of both pathogens and commensal microbiota with their animal and plant hosts.

    • Aaron T. Whiteley
    • , James B. Eaglesham
    • , Carina C. de Oliveira Mann
    • , Benjamin R. Morehouse
    • , Brianna Lowey
    • , Eric A. Nieminen
    • , Olga Danilchanka
    • , David S. King
    • , Amy S. Y. Lee
    • , John J. Mekalanos
    •  & Philip J. Kranzusch
    Nature, 1-6
  • Research | | open

    Nai-Chi Chen et al. solved the structures of two shrimp nodaviruses, focusing on the major domains to improve understanding of capsid organization. By combining cryo-EM and x-ray crystallography, the authors were able to observe the structures at a high resolution.

    • Nai-Chi Chen
    • , Masato Yoshimura
    • , Naoyuki Miyazaki
    • , Hong-Hsiang Guan
    • , Phimonphan Chuankhayan
    • , Chien-Chih Lin
    • , Shao-Kang Chen
    • , Pei-Ju Lin
    • , Yen-Chieh Huang
    • , Kenji Iwasaki
    • , Atsushi Nakagawa
    • , Sunney I. Chan
    •  & Chun-Jung Chen
  • Research | | open

    Phosphatidylinositol phosphates (PIPs) and cholesterol are known to regulate the function of late endosomes and lysosomes (LELs). Here authors unveil a mechanism by which PI(4,5)P2/PI(3,4)P2 allosterically enhances ORP1-mediated cholesterol exit from LELs.

    • Jiangqing Dong
    • , Ximing Du
    • , Huan Wang
    • , Jue Wang
    • , Chang Lu
    • , Xiang Chen
    • , Zhiwen Zhu
    • , Zhipu Luo
    • , Li Yu
    • , Andrew J. Brown
    • , Hongyuan Yang
    •  & Jia-Wei Wu
  • Research | | open

    Lipid membrane fusion is an essential function in many biological processes but little is known about membrane fusion in prokaryotes. The authors here study how haloarchaeal pleomorphic viruses (HRPVs) infect archaeal hosts. The structure-function analysis of the spike proteins shed light on prokaryotic membrane fusion.

    • Kamel El Omari
    • , Sai Li
    • , Abhay Kotecha
    • , Thomas S. Walter
    • , Eduardo A. Bignon
    • , Karl Harlos
    • , Pentti Somerharju
    • , Felix De Haas
    • , Daniel K. Clare
    • , Mika Molin
    • , Felipe Hurtado
    • , Mengqiu Li
    • , Jonathan M. Grimes
    • , Dennis H. Bamford
    • , Nicole D. Tischler
    • , Juha T. Huiskonen
    • , David I. Stuart
    •  & Elina Roine

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