X-ray crystallography

X-ray crystallography is a technique that uses X-ray diffraction patterns to determine high-resolution, three-dimensional structures of molecules such as proteins, small organic molecules, and materials. The substance of interest must be in crystalline form, which typically requires testing various crystallization conditions.

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Latest Research and Reviews

  • Research | | open

    The leading cause of cystic fibrosis is the deletion of phenylalanine 508 (F508del) in the first nucleotide-binding domain (NBD1) of the cystic fibrosis transmembrane conductance regulator (CFTR). Here authors we develop nanobodies targeting NBD1 of human CFTR and demonstrate their ability to stabilize both isolated NBD1 and full-length protein.

    • Maud Sigoillot
    • , Marie Overtus
    • , Magdalena Grodecka
    • , Daniel Scholl
    • , Abel Garcia-Pino
    • , Toon Laeremans
    • , Lihua He
    • , Els Pardon
    • , Ellen Hildebrandt
    • , Ina Urbatsch
    • , Jan Steyaert
    • , John R. Riordan
    •  & Cedric Govaerts
  • Research | | open

    The transport of L-lactate across plasma membranes is catalyzed by proton-driven monocarboxylate transporters (MCTs) of the SLC16 solute carrier family. Here, the authors present the crystal structures of a bacterial SLC16 homologue with the bound substrate L-lactate and ligand thiosalicylate both in an outward-open conformation and discuss the L-lactate transport mechanism.

    • Patrick D. Bosshart
    • , David Kalbermatter
    • , Sara Bonetti
    •  & Dimitrios Fotiadis
  • Research | | open

    Ribonucleotide reductase (RNR) catalyzes the conversion of ribonucleotides to deoxyribonucleotides, which is an essential step in DNA synthesis. Here the authors use small-angle X-ray scattering, X-ray crystallography, and cryo-electron microscopy to capture active and inactive forms of the Bacillus subtilis RNR and provide mechanistic insights into a convergent form of allosteric regulation.

    • William C. Thomas
    • , F. Phil Brooks III
    • , Audrey A. Burnim
    • , John-Paul Bacik
    • , JoAnne Stubbe
    • , Jason T. Kaelber
    • , James Z. Chen
    •  & Nozomi Ando
  • Research | | open

    XFEL radiation is providing new opportunities for probing biological systems. Here the authors perform nanoscale x-ray imaging of microtubules with helical symmetry, by using imaging sorting and reconstruction techniques.

    • Gisela Brändén
    • , Greger Hammarin
    • , Rajiv Harimoorthy
    • , Alexander Johansson
    • , David Arnlund
    • , Erik Malmerberg
    • , Anton Barty
    • , Stefan Tångefjord
    • , Peter Berntsen
    • , Daniel P. DePonte
    • , Carolin Seuring
    • , Thomas A. White
    • , Francesco Stellato
    • , Richard Bean
    • , Kenneth R. Beyerlein
    • , Leonard M. G. Chavas
    • , Holger Fleckenstein
    • , Cornelius Gati
    • , Umesh Ghoshdastider
    • , Lars Gumprecht
    • , Dominik Oberthür
    • , David Popp
    • , Marvin Seibert
    • , Thomas Tilp
    • , Marc Messerschmidt
    • , Garth J. Williams
    • , N. Duane Loh
    • , Henry N. Chapman
    • , Peter Zwart
    • , Mengning Liang
    • , Sébastien Boutet
    • , Robert C. Robinson
    •  & Richard Neutze
  • Research | | open

    Mutant RAS family members occur in a wide range of tumour types, and there is a great interest in identifying isoform-specific inhibitors. Here, the authors characterise two designed ankyrin repeat proteins (DARPins) that specifically inhibit the KRAS isoform by binding to the region around the KRAS-specific residue histidine 95 and show that they affect KRAS/effector interactions in different ways.

    • Nicolas Bery
    • , Sandrine Legg
    • , Judit Debreczeni
    • , Jason Breed
    • , Kevin Embrey
    • , Christopher Stubbs
    • , Paulina Kolasinska-Zwierz
    • , Nathalie Barrett
    • , Rose Marwood
    • , Jo Watson
    • , Jon Tart
    • , Ross Overman
    • , Ami Miller
    • , Christopher Phillips
    • , Ralph Minter
    •  & Terence H. Rabbitts
  • Research | | open

    Kistamicin is a structurally divergent glycopeptide antibiotic (GPA) that contains a unique 15-membered A-O-B ring. Here, the authors obtained a crystal structure of the kistamicin OxyA/X-domain complex and analysed the cyclisation cascade leading to the formation of the A-O-B ring.

    • Anja Greule
    • , Thierry Izoré
    • , Dumitrita Iftime
    • , Julien Tailhades
    • , Melanie Schoppet
    • , Yongwei Zhao
    • , Madeleine Peschke
    • , Iftekhar Ahmed
    • , Andreas Kulik
    • , Martina Adamek
    • , Robert J. A. Goode
    • , Ralf B. Schittenhelm
    • , Joe A. Kaczmarski
    • , Colin J. Jackson
    • , Nadine Ziemert
    • , Elizabeth H. Krenske
    • , James J. De Voss
    • , Evi Stegmann
    •  & Max J. Cryle

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