Total internal reflection microscopy

Total internal reflection microscopy is a light microscopy technique that relies on total internal reflection of illumination light to generate an evanescent wave that extends less than 200 nm from the microscope coverslip. This allows selective excitation of fluorophores immediately adjacent to the coverslip or monitoring of the distance between the coverslip and a microscopic sphere.

Latest Research and Reviews

  • Research | | open

    The Arabidopsis CC1 protein maintains microtubule array stability and cellulose synthesis during salt stress. Here the authors show that CC1 engages microtubules via an intrinsically disordered N-terminus that suggests it controls microtubule dynamics in a similar way to the mammalian Tau protein.

    • Christopher Kesten
    • , Arndt Wallmann
    • , René Schneider
    • , Heather E. McFarlane
    • , Anne Diehl
    • , Ghazanfar Abbas Khan
    • , Barth-Jan van Rossum
    • , Edwin R. Lampugnani
    • , Witold G. Szymanski
    • , Nils Cremer
    • , Peter Schmieder
    • , Kristina L. Ford
    • , Florian Seiter
    • , Joshua L. Heazlewood
    • , Clara Sanchez-Rodriguez
    • , Hartmut Oschkinat
    •  & Staffan Persson
  • Protocols |

    This protocol describes enhanced number and brightness (eN&B), an approach that uses fluorescence fluctuation spectroscopy data to directly measure the oligomerization state and dynamics of fluorescently tagged proteins in living cells.

    • Francesco Cutrale
    • , Daniel Rodriguez
    • , Verónica Hortigüela
    • , Chi-Li Chiu
    • , Jason Otterstrom
    • , Stephen Mieruszynski
    • , Anna Seriola
    • , Enara Larrañaga
    • , Angel Raya
    • , Melike Lakadamyali
    • , Scott E. Fraser
    • , Elena Martinez
    •  & Samuel Ojosnegros
    Nature Protocols 14, 616-638
  • Research | | open

    3D super-resolution imaging of dynamic processes in live cells is still challenging, especially in a large field of view. Here the authors combine SIM with multi-angle evanescent light illumination and achieve improved lateral and axial resolution, with stack acquisition time in the range of 1–2 s.

    • Youhua Chen
    • , Wenjie Liu
    • , Zhimin Zhang
    • , Cheng Zheng
    • , Yujia Huang
    • , Ruizhi Cao
    • , Dazhao Zhu
    • , Liang Xu
    • , Meng Zhang
    • , Yu-Hui Zhang
    • , Jiannan Fan
    • , Luhong Jin
    • , Yingke Xu
    • , Cuifang Kuang
    •  & Xu Liu
  • Research | | open

    Plasma membrane tension is an important factor that regulates many key cellular processes. Here authors show that a specific dynamin-independent endocytic pathway is modulated by changes in tension via the mechano-transducer vinculin.

    • Joseph Jose Thottacherry
    • , Anita Joanna Kosmalska
    • , Amit Kumar
    • , Amit Singh Vishen
    • , Alberto Elosegui-Artola
    • , Susav Pradhan
    • , Sumit Sharma
    • , Parvinder P. Singh
    • , Marta C. Guadamillas
    • , Natasha Chaudhary
    • , Ram Vishwakarma
    • , Xavier Trepat
    • , Miguel A. del Pozo
    • , Robert G. Parton
    • , Madan Rao
    • , Pramod Pullarkat
    • , Pere Roca-Cusachs
    •  & Satyajit Mayor
  • Research | | open

    Processive chitinase is a linear molecular motor which moves on the surface of crystalline chitin. Here authors use single-molecule imaging, X-ray crystallography and simulations on chitinase A (SmChiA) and show that Brownian motion along the single chitin chain is rectified forward by substrate-assisted catalysis.

    • Akihiko Nakamura
    • , Kei-ichi Okazaki
    • , Tadaomi Furuta
    • , Minoru Sakurai
    •  & Ryota Iino

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