Techniques and instrumentation

Definition

Techniques and instrumentation refers to the development of methods and tools to be used in applied physics, materials science or nanotechnology for design, synthesis, manufacturing, imaging or analytics.

Featured

Latest Research and Reviews

  • Research |

    Cryo-electron microscopy analysis of yeast Rad26 bound to RNA polymerase II provides insight into the initiation of the transcription-coupled DNA repair mechanism in eukaryotes.

    • Jun Xu
    • , Indrajit Lahiri
    • , Wei Wang
    • , Adam Wier
    • , Michael A. Cianfrocco
    • , Jenny Chong
    • , Alissa A. Hare
    • , Peter B. Dervan
    • , Frank DiMaio
    • , Andres E. Leschziner
    •  & Dong Wang
  • Research | | open

    Eukaryotic genomes are partitioned into self-interacting modules or topologically associated domains (TADs) that exist at the kilo-megabase scale. Here Cattoni et al. combine super-resolution microscopy with DNA-labeling methods to quantify absolute frequencies of interactions within TADs.

    • Diego I. Cattoni
    • , Andrés M. Cardozo Gizzi
    • , Mariya Georgieva
    • , Marco Di Stefano
    • , Alessandro Valeri
    • , Delphine Chamousset
    • , Christophe Houbron
    • , Stephanie Déjardin
    • , Jean-Bernard Fiche
    • , Inma González
    • , Jia-Ming Chang
    • , Thomas Sexton
    • , Marc A. Marti-Renom
    • , Frédéric Bantignies
    • , Giacomo Cavalli
    •  & Marcelo Nollmann
  • Research | | open

    Target of rapamycin (TOR) kinase operates within two distinct multiprotein complexes named TORC1 and TORC2. Here the authors report a cryo-EM structure of TORC2, establish its subunit organization, providing a rationale for TORC2’s rapamycin insensitivity and the mutually exclusive inclusion of Avo3/Rictor or Raptor within their respective TOR complex.

    • Manikandan Karuppasamy
    • , Beata Kusmider
    • , Taiana M. Oliveira
    • , Christl Gaubitz
    • , Manoel Prouteau
    • , Robbie Loewith
    •  & Christiane Schaffitzel
  • Research | | open

    The transmembrane receptor integrin is activated by talin, but so far it has remained elusive how talin is recruited to the plasma membrane. Here, the authors identify the Rap1-mediated membrane-targeting mechanism for talin, present the Rap1b/talin-F0 structure and show that talin is a direct Rap1b effector.

    • Liang Zhu
    • , Jun Yang
    • , Thomas Bromberger
    • , Ashley Holly
    • , Fan Lu
    • , Huan Liu
    • , Kevin Sun
    • , Sarah Klapproth
    • , Jamila Hirbawi
    • , Tatiana V. Byzova
    • , Edward F. Plow
    • , Markus Moser
    •  & Jun Qin
  • Research | | open

    Exploring the plasma processes in the pre-plasma state that lead to instabilities is challenging. Here the authors probe the evolution of the plasma phase change and the instabilities in plasma created by an exploding copper wire in Z-pinch geometry using shadowgraphy.

    • E. Kaselouris
    • , V. Dimitriou
    • , I. Fitilis
    • , A. Skoulakis
    • , G. Koundourakis
    • , E. L. Clark
    • , Μ. Bakarezos
    • , I. K. Nikolos
    • , N. A. Papadogiannis
    •  & M. Tatarakis
  • Research | | open

    Nearly all atmospheric aerosols contain surface-active organic compounds; however, the nature of how they arrange remains poorly understood. Here, the authors show that fatty acids in atmospheric aerosol proxies self-assemble into highly ordered, viscous 3D nanostructures that undergo changes upon exposure to humidity and ozone.

    • C. Pfrang
    • , K. Rastogi
    • , E. R. Cabrera-Martinez
    • , A. M. Seddon
    • , C. Dicko
    • , A. Labrador
    • , T. S. Plivelic
    • , N. Cowieson
    •  & A. M. Squires

News and Comment

  • News and Views |

    The 'off-targets' of a drug are often poorly characterized yet could be harnessed in the treatment of complex diseases. A recent study used a small-molecule screening in non-small-cell lung cancer to repurpose an FDA-approved ALK/IGF1R inhibitor and uncover its mechanism of action.

    • Gaye Saginc
    • , Franziska Voellmy
    •  & Rune Linding
    Nature Chemical Biology 13, 1204–1205
  • News and Views |

    The microstate of geometrically frustrated two-dimensional arrays of strongly interacting nanomagnets is controlled by means of topological defect-driven magnetic writing, to the extent that elusive configurations such as the ground state and negative-temperature states are realized.

    • Cristiano Nisoli
  • News and Views |

    Assembly of the small ribosomal subunit from an RNA strand and 33 proteins is an intricate and dynamic process. Two cryo-EM studies now provide insight into a complicated complex of at least 51 trans-factors that act on the preribosomal small subunit to sequentially fold it into a 3D molecular machine.

    • Joanna Rorbach
    • , Shintaro Aibara
    •  & Alexey Amunts