Super-resolution microscopy articles from across Nature Portfolio

Electrochemical control of the switching of fluorophores in stochastic optical reconstruction microscopy (EC-STORM) enables the counting of single fluorophores as well as cell imaging with improved spatial resolution and reduced artefacts compared with traditional STORM.

Wide-field mid-infrared photothermal imaging is developed to supress the resolution degradation caused by photo-thermal heat diffusion. By employing a single-objective synthetic-aperture imaging with synchronized subnanosecond mid-infrared and visible light sources, spatial resolution of 120 nm is obtained.

A pretrained foundation model (UniFMIR) enables versatile and generalizable performance across diverse fluorescence microscopy image reconstruction tasks.

The combination of light sheet illumination and reversibly switchable fluorophores enables improved structured illumination microscopy for fast, low-background super-resolution imaging in cells and spheroids.

Using single-molecule tracking and spatiotemporal mapping, Ling et al. show that the C-terminal domain of RNA polymerase II facilitates its dynamic confinement in subnuclear regions enriched in active genes, where it promotes targeted transcription.

Cancer cells adjust the composition of their glycocalyx to increase its thickness and create a physical barrier that shields them from immune recognition and engagement.

We introduce GelMap, a flexible workflow for reporting deformations and anisotropy in expansion microscopy. By intrinsically calibrating the expansion hydrogel using a fluorescent grid that scales with expansion and deforms with anisotropy, GelMap enables the reliable quantification of expansion factors and correction of deformations.

An expansion microscopy technique called ChromExM offers detailed views into the organization chromatin and associated gene expression machinery in embryos.

The introduction of a two-step deconvolution workflow maximizes the detection of fluorescence in fluctuation-based super-resolution imaging, enabling a square millimetre field of view to be captured in as little as ten minutes.